A tag-free fluorescent aptasensor for ultrasensitive detection of sulfadimethoxine antibiotic mediated by rolling circle amplified DNA cascades

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-28 DOI:10.1016/j.saa.2025.126309

Yasaman Solouki , Zahra Khoshbin , Abouzar Shafaghi , Mohammad Ramezani , Mona Alibolandi , Khalil Abnous , Seyed Mohammad Taghdisi

{"title":"A tag-free fluorescent aptasensor for ultrasensitive detection of sulfadimethoxine antibiotic mediated by rolling circle amplified DNA cascades","authors":"Yasaman Solouki , Zahra Khoshbin , Abouzar Shafaghi , Mohammad Ramezani , Mona Alibolandi , Khalil Abnous , Seyed Mohammad Taghdisi","doi":"10.1016/j.saa.2025.126309","DOIUrl":null,"url":null,"abstract":"<div><div>Elevated concentrations of sulfadimethoxine (SDM) in food products contribute to the development of bacterial resistance, resulting in a range of health problems for individuals. This background makes it incumbent to develop an efficient and accurate detection method for SDM. Rolling circle amplification (RCA) is an easy operation and highly efficient signal improvement technique that does not require thermal cycling. This study presents a fluorescent aptasensor that operates without labels to detect SDM, utilizing the RCA process. Using the RCA method as the main contributor for signal amplification, a large number of DNA copies is produced in a short time. This allows for the immobilization of SYBR Green (SGI) molecules and a sharp increase in the fluorescence response. Hence, it can be achieved a wide detection range of SDM from 1 pM to 200 nM and an impressive detection limit of 0.238 pM. The aptasensor can successfully monitor SDM in various livestock products and biological samples. This aptasensor offers advantages such as a short fabrication time, cost-effectiveness, and user-friendliness. It can be promoted as a convenient and economic portable testing kit.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"339 ","pages":"Article 126309"},"PeriodicalIF":4.3000,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142525006158","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}

引用次数: 0

Abstract

Elevated concentrations of sulfadimethoxine (SDM) in food products contribute to the development of bacterial resistance, resulting in a range of health problems for individuals. This background makes it incumbent to develop an efficient and accurate detection method for SDM. Rolling circle amplification (RCA) is an easy operation and highly efficient signal improvement technique that does not require thermal cycling. This study presents a fluorescent aptasensor that operates without labels to detect SDM, utilizing the RCA process. Using the RCA method as the main contributor for signal amplification, a large number of DNA copies is produced in a short time. This allows for the immobilization of SYBR Green (SGI) molecules and a sharp increase in the fluorescence response. Hence, it can be achieved a wide detection range of SDM from 1 pM to 200 nM and an impressive detection limit of 0.238 pM. The aptasensor can successfully monitor SDM in various livestock products and biological samples. This aptasensor offers advantages such as a short fabrication time, cost-effectiveness, and user-friendliness. It can be promoted as a convenient and economic portable testing kit.

Abstract Image

查看原文本刊更多论文

滚动环扩增DNA级联介导的磺胺二甲氧嘧啶抗生素超灵敏检测的无标记荧光适体传感器

食品中磺胺二甲氧嘧啶（SDM）浓度的升高有助于细菌耐药性的发展，从而给个人带来一系列健康问题。在这样的背景下，开发一种高效、准确的SDM检测方法势在必行。滚动圈放大（RCA）是一种操作简单、不需要热循环的高效信号改进技术。本研究提出了一种荧光适体传感器，该传感器使用RCA过程，无需标记即可检测SDM。利用RCA方法作为信号放大的主要贡献者，可以在短时间内产生大量的DNA拷贝。这允许SYBR绿色（SGI）分子的固定化和荧光响应的急剧增加。因此，它可以实现从1 pM到200 nM的宽SDM检测范围和令人印象深刻的0.238 pM的检测限。该传感器可以成功地监测各种畜产品和生物样品中的SDM。该传感器具有制造时间短、成本效益高、用户友好等优点。它可以作为一种方便、经济的便携式检测试剂盒进行推广。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.