Molecular insights into the mechanism of white syndrome in Macrobrachium rosenbergii through transcriptome analysis

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-04-21 DOI:10.1016/j.aquaculture.2025.742605

Feiyang Wang , Jingyi Liu , Jianhu Jiang , Haiqi Zhang , Jiasong Xie , Jianlin Guo , Lihui Sun , Qian Li , Yunpeng Fan , Jing Chen

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Abstract

In China, a newly emerging disease, White Syndrome (WS), has been identified in Macrobrachium rosenbergii. This study investigates the impact of WS, characterized by systemic whitening, abdominal muscle and hepatopancreatic atrophy, and the presence of blisters. Histopathological and transcriptomic analyses were performed on hepatopancreas and abdominal muscle tissues of affected prawns. Histological examinations revealed damaged hepatopancreatic tubules with enlarged intercellular spaces, while muscle tissues exhibited signs of atrophy. A total of 191 differentially expressed genes (DEGs) were identified from the hepatopancreas, along with 233 KEGG pathways. The primary pathways associated with WS include Lysosome, Glycosaminoglycan degradation, Pentose and glucuronate interconversions, Starch and sucrose metabolism, Biosynthesis of unsaturated fatty acids, Sphingolipid metabolism, Steroid biosynthesis, Fatty acid elongation and Retinol metabolism. Similarly, 191 DEGs were identified from the abdominal muscle, with 203 KEGG pathways. WS-related pathways included Proteasome, Glycolysis/Gluconeogenesis, and Starch and sucrose metabolism. These findings suggest that WS in M. rosenbergii may be associated with nutritional deficiencies linked to excessive reproductive activity in female prawns. This study provides critical insights for future research on WS and its management in M. rosenbergii.

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罗氏沼虾白色综合征发生机制的转录组分析

在中国，罗氏沼虾（Macrobrachium rosenbergii）中发现了一种新的疾病——白综合征（White Syndrome， WS）。本研究探讨WS的影响，其特点是全身变白，腹肌和肝胰腺萎缩，并存在水疱。对患病对虾的肝胰腺和腹肌组织进行了组织病理学和转录组学分析。组织学检查显示肝胰腺小管受损，细胞间隙增大，肌肉组织萎缩。从肝胰腺中共鉴定出191个差异表达基因（deg），以及233个KEGG通路。与WS相关的主要途径包括溶酶体、糖胺聚糖降解、戊糖和葡萄糖酸盐相互转化、淀粉和蔗糖代谢、不饱和脂肪酸的生物合成、鞘脂代谢、类固醇生物合成、脂肪酸延伸和视黄醇代谢。同样，从腹肌中鉴定出191个deg，有203个KEGG通路。ws相关途径包括蛋白酶体、糖酵解/糖异生、淀粉和蔗糖代谢。这些发现表明，罗氏沼虾的WS可能与雌性对虾过度繁殖活动引起的营养缺乏有关。本研究为今后对罗氏m.r osbergii的WS及其管理的研究提供了重要的见解。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.