Engineering strategies for producing medium-long chain dicarboxylic acids in oleaginous yeasts

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-04-26 DOI:10.1016/j.biortech.2025.132593

Xin Wang , Mei-Li Sun , Lu Lin , Rodrigo Ledesma-Amaro , Kaifeng Wang , Xiao-Jun Ji

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Abstract

Medium-long chain dicarboxylic acids (DCAs, C ≥ 6) are essential chemical raw materials, with wide applications in the chemical, pharmaceutical, material and food industries. However, the traditional chemical synthesis methods cause environmental pollution and are not in line with goals of sustainable development. With the development of synthetic biology, high-value-added DCAs can be biosynthesized from hydrophobic substrates (HSs) using suitable microorganisms. This review first summarizes the biosynthetic pathway of DCAs in oleaginous yeasts and then emphasizes the related engineering strategies for increasing the product yield, including promoter, enzyme, pathway, cell, fermentation, and downstream engineering. In addition, the challenges and development trends in the biosynthesis of DCAs are discussed, in light of the current progress, challenges, and trends in this field. Finally, guidelines for future research are proposed. Overall, this review systematically summarizes recent engineering strategies for DCAs production in oleaginous yeasts and offers valuable insights for future DCAs biosynthesis.

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产油酵母生产中长链二羧酸的工程策略

中长链二羧酸（DCAs, C≥6）是重要的化工原料，在化工、制药、材料、食品等行业有着广泛的应用。然而，传统的化学合成方法造成环境污染，不符合可持续发展的目标。随着合成生物学的发展，利用合适的微生物从疏水底物（HSs）合成高附加值的DCAs成为可能。本文首先综述了DCAs在产油酵母中的生物合成途径，重点介绍了提高产物产量的相关工程策略，包括启动子、酶、途径、细胞、发酵和下游工程。此外，根据目前DCAs生物合成的进展、挑战和趋势，讨论了DCAs生物合成面临的挑战和发展趋势。最后，提出了今后研究的指导方针。综上所述，本文系统总结了近年来产油酵母生产DCAs的工程策略，并为未来DCAs的生物合成提供了有价值的见解。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.