A simplified one-pot derivatization/magnetic solid-phase extraction with integrated pH adjustment strategy coupled with liquid chromatography-fluorescence detection for fatty aldehyde analysis

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2025-04-24 DOI:10.1016/j.chroma.2025.465988

Nian Shi , Yuyang Wang , Mengyuan Lv , Xudong Xia , Zihan Li , Di Chen , Ranran Duan , Jinghua Zhang

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引用次数: 0

Abstract

A novel one-pot derivatization/magnetic solid-phase extraction with integrated pH adjustment (OPD/MSPE-pH) strategy was developed for the efficient and selective quantification of fatty aldehydes. This method seamlessly integrates pH regulation, derivatization, and magnetic separation in a single incubation step, where the sample solution, derivatization reagent, and magnetic composite (Fe₃O₄/MWCNTs-OH/CA) are processed together, significantly simplifying sample preparation. The magnetic composite, synthesized from Fe₃O₄ nanoparticles, hydroxylated multi-walled carbon nanotubes (MWCNTs-OH), and citric acid (CA), functions both as an acidic pH regulator and an efficient adsorbent for aldehyde-fluorenylmethyl carbazate (FMC) derivatives. Compared to conventional approaches, the OPD/MSPE-pH method exhibited superior operation efficiency, achieving low limits of detection (1.1–2.4 nM) and high recoveries (91.7–106.1 %). The optimized procedure was successfully applied to plasma and spiked plasma samples, demonstrating its effectiveness in complex biological matrices. This streamlined technique eliminates the need for additional pH-adjusting reagents and centrifugation, offering a practical, rapid, and highly efficient solution for fatty aldehyde analysis. Overall, the proposed OPD/MSPE-pH method provides a robust, sensitive, and reliable analytical platform for fatty aldehyde analysis, with potential applications in broader fields with suitable modifications.

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一种简化的一锅衍生/磁固相萃取集成pH调整策略-液相色谱-荧光检测法分析脂肪醛

建立了一种新型的一锅衍生/磁固相萃取集成pH调节（OPD/MSPE-pH）策略，用于高效、选择性地定量脂肪醛。该方法将pH调节、衍生化和磁分离无缝集成在一个孵育步骤中，其中样品溶液、衍生化试剂和磁性复合物（Fe₃O₄/MWCNTs-OH/CA）一起处理，大大简化了样品制备。由Fe₃O₄纳米颗粒、羟基化多壁碳纳米管（MWCNTs-OH）和柠檬酸（CA）合成的磁性复合材料，既可以作为酸性pH调节剂，又可以作为醛-氟酰基甲基氨基甲酸酯（FMC）衍生物的高效吸附剂。与传统方法相比，OPD/MSPE-pH法具有较好的操作效率，检出限低（1.1 ~ 2.4 nM），回收率高（91.7 ~ 106.1%）。优化后的程序成功地应用于血浆和加标血浆样品，证明了其在复杂生物基质中的有效性。这种流线型技术消除了额外的ph调整试剂和离心的需要，为脂肪醛分析提供了实用、快速、高效的解决方案。综上所述，所提出的OPD/MSPE-pH方法为脂肪醛分析提供了一个稳健、灵敏、可靠的分析平台，通过适当的修改，具有更广泛的应用前景。

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.