Biofabrication of nano-silver composites from Indian catmint-Anisomeles ovata flower buds extract and evaluation of their potential in-vitro biological applications

Pharmacological Research - Natural Products Pub Date : 2025-04-25 DOI:10.1016/j.prenap.2025.100246

Shashiraj Kariyellappa Nagaraja , Bidhayak Chakraborty , Anil H , Meghashyama Prabhakara Bhat , Sreenivasa Nayaka

{"title":"Biofabrication of nano-silver composites from Indian catmint-Anisomeles ovata flower buds extract and evaluation of their potential in-vitro biological applications","authors":"Shashiraj Kariyellappa Nagaraja , Bidhayak Chakraborty , Anil H , Meghashyama Prabhakara Bhat , Sreenivasa Nayaka","doi":"10.1016/j.prenap.2025.100246","DOIUrl":null,"url":null,"abstract":"<div><div>This research examines the phyto-fabrication of silver nanoparticles utilizing the extract from <em>Anisomeles ovata</em> flower buds, along with their potential applications in antimicrobial and anticancer activities. The synthesized AoFb-AgNPs underwent characterisation using a range of bio-analytical techniques. The synthesis was validated via UV-Vis spectrophotometry, revealing an absorption peak at 408 nm. The FTIR analysis confirmed the reduction and capping of AoFb-AgNPs, revealing peaks associated with different functional groups. The examinations conducted using SEM-EDS and TEM indicated that the AoFb-AgNPs exhibited a spherical morphology, while the EDS analysis validated the presence of silver atoms at an energy level of 3 keV. The crystalline structure of AoFb-AgNPs was elucidated through XRD studies. The size and stability of the synthesized AgNPs were determined through DLS and Zeta potential tests, resulting in a measurement of 99.7 nm. The TGA analyses of AoFb-AgNPs exhibited considerable stability across the various tested temperatures, indicating minimal weight loss. AoFb-AgNPs produced through biological synthesis exhibited strong inhibitory effects on various microbial strains. Additionally, the effectiveness of biosynthesized AoFb-AgNPs in inhibiting the growth of cancer cells was evaluated towards the Panc-1 cell line employing the MTT method. The results demonstrated that the viability of cells decreased in a manner that was dependent on the concentration. The flow cytometry analysis revealed significant percentages of cells in both early and late stages of apoptosis. This indicates that the biologically synthesized AoFb-AgNPs have the potential to be used in therapeutics to enhance their effectiveness due to their notable antimicrobial, and cytotoxic properties. Additional investigation has the potential to open up new possibilities for the creation of innovative and secure treatments for cancer, utilizing biofabricated AgNPs.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100246"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Natural Products","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950199725001065","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

This research examines the phyto-fabrication of silver nanoparticles utilizing the extract from Anisomeles ovata flower buds, along with their potential applications in antimicrobial and anticancer activities. The synthesized AoFb-AgNPs underwent characterisation using a range of bio-analytical techniques. The synthesis was validated via UV-Vis spectrophotometry, revealing an absorption peak at 408 nm. The FTIR analysis confirmed the reduction and capping of AoFb-AgNPs, revealing peaks associated with different functional groups. The examinations conducted using SEM-EDS and TEM indicated that the AoFb-AgNPs exhibited a spherical morphology, while the EDS analysis validated the presence of silver atoms at an energy level of 3 keV. The crystalline structure of AoFb-AgNPs was elucidated through XRD studies. The size and stability of the synthesized AgNPs were determined through DLS and Zeta potential tests, resulting in a measurement of 99.7 nm. The TGA analyses of AoFb-AgNPs exhibited considerable stability across the various tested temperatures, indicating minimal weight loss. AoFb-AgNPs produced through biological synthesis exhibited strong inhibitory effects on various microbial strains. Additionally, the effectiveness of biosynthesized AoFb-AgNPs in inhibiting the growth of cancer cells was evaluated towards the Panc-1 cell line employing the MTT method. The results demonstrated that the viability of cells decreased in a manner that was dependent on the concentration. The flow cytometry analysis revealed significant percentages of cells in both early and late stages of apoptosis. This indicates that the biologically synthesized AoFb-AgNPs have the potential to be used in therapeutics to enhance their effectiveness due to their notable antimicrobial, and cytotoxic properties. Additional investigation has the potential to open up new possibilities for the creation of innovative and secure treatments for cancer, utilizing biofabricated AgNPs.

查看原文本刊更多论文

印度薄荷-蛋形茴香花蕾提取物纳米银复合材料的生物制备及其体外生物学应用前景评价

本研究探讨了利用卵形茴香花蕾提取物制备纳米银的植物，以及它们在抗菌和抗癌活性方面的潜在应用。使用一系列生物分析技术对合成的AoFb-AgNPs进行了表征。通过紫外可见分光光度法对合成进行了验证，发现在408 nm处有一个吸收峰。FTIR分析证实了AoFb-AgNPs的减少和封顶，揭示了与不同官能团相关的峰。SEM-EDS和TEM分析表明，AoFb-AgNPs呈球形形貌，而EDS分析证实了银原子在3 keV能级的存在。通过XRD表征了AoFb-AgNPs的晶体结构。通过DLS和Zeta电位测试确定了合成的AgNPs的尺寸和稳定性，测量结果为99.7 nm。TGA分析显示，AoFb-AgNPs在不同的测试温度下具有相当大的稳定性，表明重量损失最小。通过生物合成制备的AoFb-AgNPs对多种微生物具有较强的抑制作用。此外，采用MTT法对生物合成的AoFb-AgNPs在Panc-1细胞株上抑制癌细胞生长的有效性进行了评价。结果表明，细胞活力随浓度的增加而降低。流式细胞术分析显示，早期和晚期细胞凋亡的比例都很显著。这表明生物合成的AoFb-AgNPs由于其显著的抗菌和细胞毒性，具有用于治疗的潜力，以提高其有效性。进一步的研究有可能为利用生物制造的AgNPs创造创新和安全的癌症治疗开辟新的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pharmacological Research - Natural Products

自引率

0.00%

发文量