Sodium alginate hydrogel platelet rich plasma exosome loaded for synergistic hemostasis

Abstract

Uncontrollable traumatic bleeding caused by surgery or traffic accidents is an important factor leading to high mortality rates. Therefore, there is an urgent hemostatic materials with fast, effective, and safe to achieve rapid hemostasis. Traditional hemostatic materials have limited effect in deep irregular tissues or massive bleeding scenes, while exocrine hydrogel can break through limitations by quickly stopping bleeding and reducing secondary injury. The gelatin time of an injectable 10 % oxidized sodium alginate-10 % gelatin (OSA-G) hydrogel was 8 s. Platelet-rich plasma exosomes (PRP-Exo) from the autologous biological macromolecule play a crucial role in blood coagulation and hemostasis. The OSA-G hydrogel loaded with PRP-Exo exhibited superior effects on proliferation and angiogenesis in vitro. The APTT and thromboelastography of the OSA-G-EXO group were significantly reduced, indicating the occurrence of intrinsic coagulation pathways and the blood was in a hypercoagulable state and promoting hemostasis. Meanwhile, it has been confirmed in the rat tail-cutting model and the liver bleeding model that the hemostasis time is approximately 30 s, promoting skin healing in the skin wound model. By providing a controlled environment for coagulopathy or anticoagulant-treated patients, it shows a good clinical application prospect.