Bioengineered poplar fibres via PagGLR2.8 editing: A synergistic design for high-performance biocomposites

IF 10.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Journal Pub Date : 2025-04-29 DOI:10.1111/pbi.70115

Yi An, Shi-Qi Wang, Xin-Yi Jia, Xue Jiao, Mei-Qiao Qu, Yan Dong, Zhong-Yuhan Wang, Zhong-Yi Ma, Song Yang, Xiao Han, Li-Chao Huang, Ning-Ning Chen, Cheng Jiang, Meng-Zhu Lu, Jin-Feng Dai, Jin Zhang

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引用次数: 0

Abstract

The urgent need to replace petroleum-derived materials with sustainable alternatives drives innovation at the nexus of plant biotechnology and materials science. Here, we engineered Populus alba × P. glandulosa ‘84 K’ through CRISPR-Cas9-mediated knockout of PagGLR2.8, a glutamate receptor gene regulating vascular development, to investigate its role in fibre biosynthesis and composite performance. Knockout of PagGLR2.8 improved the quality of poplar fibre by altering the structure and development mode of poplar vascular tissue. Our study established the relationship between fibre quantity and structure and the performance of polylactic acid (PLA) composites. The mechanical and fire-resistance properties of these transgenic plant fibres/PLA composites significantly outperformed those of pure PLA, demonstrating the potential of phloem fibres to reinforce toughened composites. Notably, we also evaluated flammability and dripping behaviours, with findings indicating that our optimised fibre/PLA composites exhibit superior strengths, modulus, fire resistance, and anti-dripping, surpassing those of PLA. This research unveils a groundbreaking approach to regulating composite properties through genetic manipulation and highlights the promising potential of plant-derived materials in enriching forest resources and advancing the sustainable utilisation of poplar fibres and polymers.

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通过PagGLR2.8编辑的生物工程杨树纤维：高性能生物复合材料的协同设计

迫切需要用可持续的替代品取代石油衍生材料，这推动了植物生物技术和材料科学之间的创新。本研究通过crispr - cas9介导敲除谷氨酸受体基因PagGLR2.8，对白杨（Populus alba × P. glandullosa） 84 K进行基因工程改造，研究其在纤维生物合成和复合性能中的作用。敲除PagGLR2.8基因通过改变杨树维管组织的结构和发育模式，提高了杨树纤维的质量。本研究建立了聚乳酸（PLA）复合材料纤维数量、结构与性能之间的关系。这些转基因植物纤维/PLA复合材料的力学性能和耐火性能明显优于纯PLA，表明韧皮部纤维增强增韧复合材料的潜力。值得注意的是，我们还评估了可燃性和滴水行为，结果表明，我们优化的纤维/PLA复合材料具有优越的强度、模量、防火性和防滴水性，超过了PLA。这项研究揭示了一种突破性的方法，通过基因操纵来调节复合材料的特性，并强调了植物源材料在丰富森林资源和推进杨树纤维和聚合物的可持续利用方面的巨大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Biotechnology Journal 生物-生物工程与应用微生物

CiteScore

20.50

自引率

2.90%

发文量

201

审稿时长

1 months

期刊介绍： Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.