Cell membrane biomimetic nanoparticle-enhanced SPR biosensor for facile and sensitive detection of circulating tumor cells

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-04-28 DOI:10.1016/j.snb.2025.137872

Yawen Liu , Hezhen Liu , Wenyi Yang , Chengcheng Xu , Qiang Xie , Hongzhi Song , Xiaojun Hu , Hongxia Chen

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引用次数: 0

Abstract

Circulating tumor cells (CTCs) released from solid tumors into the peripheral blood are valuable in assessing the risk of breast cancer metastasis and guiding precision therapy. This study proposes a surface plasmon resonance (SPR) strategy utilizing functionalized red blood cell membranes (RBCM-PPP) and CTCs membranes functionalized gold nanoparticles (AuNPs@Mem) for the highly sensitive detection of CTCs. The RBCM-PPP was modified on the surface of the gold chip by sulfhydryl groups on DSPE-PEG-SH, and the ERBB2 protein expressed on the membrane surface of CTCs specifically bound to the ERBB2 palmitoylated polypeptide (PP) in the RBCM-PPP, while the AuNPs in AuNPs@Mem acted as signal amplifying molecules to achieve the sensitive detection of AuNPs@Mem. The constructed biosensor achieved a linear detection range of 5 × 10³ to 1 × 10⁶ particles/mL, with a detection limit as low as 868.11 particles/mL. This novel biosensing strategy possesses excellent selectivity, sensitivity, and stability, and offers potential applications for clinical CTCs detection.

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膜仿生纳米粒子增强的SPR生物传感器，用于快速灵敏地检测循环肿瘤细胞

循环肿瘤细胞（CTCs）从实体瘤释放到外周血中，在评估乳腺癌转移风险和指导精确治疗方面具有重要价值。本研究提出了一种利用功能化红细胞膜（RBCM-PPP）和ctc膜功能化金纳米粒子（AuNPs@Mem）的表面等离子体共振（SPR）策略，用于高灵敏度检测ctc。RBCM-PPP在金芯片表面被DSPE-PEG-SH上的巯基修饰，ctc膜表面表达的ERBB2蛋白与RBCM-PPP中ERBB2棕榈酰化多肽（PP）特异性结合，AuNPs@Mem中的AuNPs作为信号扩增分子，实现AuNPs@Mem的灵敏检测。所构建的生物传感器线性检测范围为5×103 ~ 1×106 particles/mL，检出限低至868.11 particles/mL。这种新型的生物传感策略具有良好的选择性、灵敏度和稳定性，在临床ctc检测中具有潜在的应用前景。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.