Innovative two-dimensional fluorescent probe of viscosity and ONOO- for high-throughput screening of antioxidant drugs and its application in epilepsy

Abstract

Epilepsy is a neurodegenerative disease closely associated with oxidative stress and neuronal damage, posing a grave threat to human health. Unfortunately, the prompt diagnosis and effective treatment of epilepsy currently remain a substantial challenge. Here, a novel two-dimensional fluorescent probe, NIR-VO, has been rationally developed for the detection of LDs viscosity and ONOO^- changes in epilepsy models. It is worth noting that the high-throughput screening of common antioxidants has been achieved using the probe's non-interfering dual-channel feedback signals, and the experimental results indicate that melatonin has the optimal antioxidant stress response and neuroprotective effects. More significantly, melatonin has also exhibited tremendous antiepileptic potential in the imaging of cell, zebrafish and mice epilepsy models. It is expected that this work will provide new insights for the construction of innovative multidimensional fluorescent probes, and boost the advancement of diagnosis and treatment research for epilepsy and related neurological diseases.