2A-biohydrogels accelerate diabetic wound healing by promoting M2 macrophage polarization and functionalized mitochondrial transfer to endothelial cells

IF 13.3 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-04-28 DOI:10.1016/j.cej.2025.163130

Hao Wang , Min Zhou , Yifeng Ruan , Yifei Shen , Ziqi Qin , Xingbo Wu , Huiling Ling , Wushuang Ye , Yongfu Wang , Xueqi Gan

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Abstract

Diabetic chronic wounds pose a significant clinical challenge due to their complex pathophysiology and limited treatment options. In this study, a silk fibroin/gelatin (SG) gel incorporated with Mdivi-1 (SG/M) was developed and applied to chronic diabetic wounds. The SG/M hydrogel significantly enhanced diabetic wound healing compared to the plain SG hydrogel. This potential therapeutic potential of Mdivi-1 relies on promoting macrophage polarization toward the M2 phenotype and restoring hyperglycemia-mediated mitochondrial dynamic disorders and dysfunction in both macrophages and human umbilical vein endothelial cells (HUVECs) under high glucose conditions. Furthermore, Mdivi-1 facilitated mitochondrial transfer from macrophages to HUVECs, which further enhanced mitochondrial function in HUVECs and improved their cellular activity. These findings not only establish Mdivi-1 as a potential novel therapy but also provide a theoretical foundation for targeting diabetic complications, underscoring the originality and clinical relevance of this research.

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2a -生物水凝胶通过促进M2巨噬细胞极化和功能化线粒体向内皮细胞转移来加速糖尿病伤口愈合

糖尿病性慢性伤口由于其复杂的病理生理和有限的治疗选择而构成了重大的临床挑战。本研究将丝素/明胶（SG）凝胶掺入Mdivi-1 (SG/M)，并应用于慢性糖尿病创面。与普通SG水凝胶相比，SG/M水凝胶显著促进糖尿病伤口愈合。Mdivi-1的潜在治疗潜力依赖于促进巨噬细胞向M2表型极化，并在高糖条件下恢复巨噬细胞和人脐静脉内皮细胞（HUVECs）中高血糖介导的线粒体动力学紊乱和功能障碍。此外，Mdivi-1促进了巨噬细胞向HUVECs的线粒体转移，进一步增强了HUVECs的线粒体功能，提高了HUVECs的细胞活性。这些发现不仅确立了Mdivi-1作为一种潜在的新疗法，而且为针对糖尿病并发症提供了理论基础，突出了本研究的独创性和临床意义

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.