Transcriptional responses of Mediterranean mussels (Mytilus galloprovincialis) under the 2022 Marine Heatwave: a trade-off of physiological regulation between metabolism, stress response, and shell biomineralization in a mixed exposure scenario

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2025-04-28 DOI:10.1016/j.envpol.2025.126328

Letizia Iuffrida , Silvia Franzellitti

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Abstract

There has been a notable increase in occurrence and intensity of marine heatwaves (MHWs) over the past decades, with a consequent remarkable risk to vulnerable species as marine bivalves. This study examines the responses of farmed Mytilus galloprovincialis to the 2022 MHW that impacted the Northwestern (NW) Adriatic Sea. Expression of key transcripts involved in functions of digestive glands and mantles were investigated to explore the putative acclimatory processes contributing to mussel fitness. The 2022 MHW was characterized by persistent sea temperature anomalies, elevated salinity, and dramatically low chlorophyll-a levels. Despite the temporal trends of pH and the extreme seawater temperatures reached in July and August, the carbonate system never reached the undersaturation state, being favourable for bivalve biomineralization. Transcriptional profiles in digestive glands and mantles displayed a two-step temporal response. In digestive glands, metabolism and lysosomal response functional categories showed an initial decrease (late May), and a recovery in late August. Antioxidant and cytoprotective related gene products showed a February to August increased expression, with strong up-regulations in August. In mantles, transcripts involved in shell biomineralization were prompted in the initial stage of the MHW, likely to withstand the abrupt changes of seawater parameters and to maintain bivalve growth. At high MHW intensities, energy was diverted towards the strong stress response activation in digestive glands, with a relative decrease of mRNA levels for shell biomineralization transcripts. Results showed that a trade-off between core physiological processes may contribute to the acclimatory response of mussels to cope with the adverse conditions of the 2022 MHW in the NW Adriatic Sea.

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2022年海洋热浪下地中海贻贝（Mytilus galloprovincialis）的转录反应：混合暴露情景下代谢、应激反应和贝壳生物矿化之间生理调节的权衡

在过去的几十年里，海洋热浪（MHW）的发生和强度显著增加，从而给海洋双壳类等脆弱物种带来了显著的风险。本研究考察了养殖的褐贻贝（Mytilus galloprovincialis）对影响西北亚得里亚海的2022年MHW的反应。研究了与消化腺和肠衣功能相关的关键转录本的表达，以探索贻贝适应的可能的适应过程。2022年MHW的特征是持续的海温异常，盐度升高，叶绿素-a水平急剧降低。尽管7、8月的pH值和海水温度呈时间变化趋势，但碳酸盐体系从未达到过欠饱和状态，有利于双壳类生物矿化。消化腺和披风的转录谱显示了两步时间反应。在消化腺中，代谢和溶酶体反应功能类别表现出最初的下降（5月下旬），8月下旬恢复。抗氧化和细胞保护相关基因产物在2 ~ 8月表达量增加，其中8月表达量上调幅度较大。在地幔中，参与壳生物矿化的转录本在MHW的初始阶段被激发，可能承受海水参数的突变并维持双壳类动物的生长。在高MHW强度下，能量被转移到消化腺的强应激反应激活上，壳生物矿化转录物的mRNA水平相对降低。结果表明，亚得里亚海贻贝对2022年MHW不利条件的适应反应可能与核心生理过程之间的权衡有关。

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.