Screening of efficient Antimony-oxidizing bacterium Lysinibacillus HC02 and its effect on preventing the absorption and accumulation of Sb in Brassica napus

IF 4.1 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2025-04-29 DOI:10.1007/s11104-025-07496-2

Zicong Xiong, Caiyuan Ling, Qian Zhang, Junwei Tang, Jingying Gu, Yuan Su, Weiwei Yang, Chaolan Zhang

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Abstract

Background and aims

The oxidation of antimony (Sb) by rhizosphere microorganisms is regarded as a strategy to mitigate the phytotoxicity of Sb(III). This study aims to isolate a highly efficient Sb-oxidizing bacterium (named HC02) from Sb-contaminated soil and to investigate the bacterium's impact on the growth of Brassica napus under practical soil conditions.

Methods

The oxidative properties of HC02 were assessed in LB liquid medium at a concentration of 0.05 mM. The Brassica napus pot experiment involved inoculating HC02 using two methods: root irrigation treatment (IR02) and root dipping treatment (DR02). The growth of Brassica napus, its Sb content, the chemical properties of the rhizosphere soil, and the chemical forms of Sb present were analyzed.

Results

The oxidation rate of 0.05 mM Sb(III) by HC02 exceeds 90% within 48 h. HC02 possesses the ability to secrete 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and indole acetic acid (IAA). The addition of HC02 significantly promoted the growth of Brassica napus. Furthermore, both IR02 and DR02 treatments resulted in a significant reduction of total Sb content in roots, with decreases of 38.7% and 41.6%, respectively. Additionally, these treatments also led to a reduction in Sb(III) content in leaves, with reductions of 7.9% and 20.2%.

Conclusions

HC02 exhibits a strong oxidizing ability towards Sb(III). The incorporation of HC02 promotes the growth of Brassica napus while effectively preventing the absorption and accumulation of Sb. This research offers a theoretical foundation for the safe utilization of Sb-contaminated soil and the secure production of vegetables.

Graphical Abstract

• Lysinibacillus HC02 is a new highly efficient antimony-oxidizing bacterium.

• Antimony toxicity of Brassica napus can be mitigate by Lysinibacillus HC02.

• The mechanisms of HC02 regulating absorption and accumulation of Sb in Brassica napus was revealed.

查看原文本刊更多论文

高效氧化锑细菌hco2赖氨酸芽孢杆菌的筛选及其对甘蓝型油菜锑吸收积累的抑制作用

背景与目的根际微生物氧化锑（Sb）被认为是减轻锑（Sb）植物毒性的一种策略。本研究旨在从硫酸污染土壤中分离出一种高效硫酸氧化菌（命名为hco2），并研究该菌在实际土壤条件下对甘蓝型油菜生长的影响。方法以甘蓝型油菜为试验材料，采用根灌（IR02）和根浸（DR02）两种方式接种hco2，在浓度为0.05 mM的LB液体培养基中测定hco2的氧化特性。分析了甘蓝型油菜的生长情况、根际土壤中锑的含量、化学性质及存在的锑的化学形态。结果hco2在48 h内对0.05 mM Sb（III）的氧化率超过90%，hco2具有分泌1-氨基环丙烷-1-羧酸（ACC）脱氨酶和吲哚乙酸（IAA）的能力。hco2的添加显著促进了甘蓝型油菜的生长。此外，IR02和DR02处理显著降低了根中总Sb含量，分别降低了38.7%和41.6%。此外，这些处理也导致叶片中Sb（III）含量降低，分别降低7.9%和20.2%。结论shco2对Sb（III）具有较强的氧化能力。hco2的掺入促进了甘蓝型油菜的生长，同时有效地阻止了Sb的吸收和积累。本研究为Sb污染土壤的安全利用和蔬菜的安全生产提供了理论基础。•hco2溶胞杆菌是一种新型高效的锑氧化菌。•赖氨酸芽孢杆菌hco2可减轻甘蓝型油菜的锑毒性。•揭示了hco2调控甘蓝型油菜Sb吸收和积累的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.