Effect of inoculation with arbuscular mycorrhizal fungi on larvicidal activity and phenolic compounds in Mimosa tenuiflora cultivated under field conditions

IF 2.5 Q2 MULTIDISCIPLINARY SCIENCES

Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2025-04-28 DOI:10.1186/s43088-025-00625-x

Joao Cleme Ananias de Sousa-Junior, Melquisedec de Sousa Oliveira, Carlos Henrique de Araújo Dias, Andréia Amariz, Maryluce Albuquerque da Silva Campos

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引用次数: 0

Abstract

Background

Mimosa tenuiflora (Willd.) is an essential leguminous tree used in Brazilian northeastern folk medicine, and its extracts have been tested against larvae of Aedes aegypti. These tests typically use parts from adult plants collected in natural environments. However, M. tenuiflora seedlings can be successfully produced using arbuscular mycorrhizal fungi (AMF) inoculation technology. Previous studies have reported the benefits of inoculation on M. tenuiflora growth and the accumulation of secondary metabolites, while data on how inoculation affects the biological activities of extracts remain limited. This work investigated the potential of field inoculation with arbuscular mycorrhizal fungi to enhance M. tenuiflora production, focusing on increasing total phenolic levels and improving the larvicidal activity of its extracts against Ae. aegypti.

Results

Inoculation with Gigaspora albida resulted in higher levels of total phenolics, death of larvae, and lower lethal concentration (LC) compared with other treatments. The concentration of phenolics was 249.87 mg. g⁻¹, and the percentage of death after 48 h was 68.33%. At 48 h, the LC₅₀ and LC₉₀ values were 147 µg. mL⁻¹ and 1301.83 µg. mL⁻¹ for extracts from plants inoculated with G. albida. For the non-inoculated controls, the LC₅₀ was 800.67 µg. mL⁻¹ and LC₉₀ 8194.26 µg. mL⁻¹, while the inoculation with Claroideoglomus etunicatum resulted in LC₅₀ 1179.16 µg. mL⁻¹ and LC₉₀ 3050.32 µg. mL⁻¹. No differences were observed in the percentage of larvae mortality between extracts from plants inoculated with C. etunicatum and non-inoculated controls. The increased concentration of total phenolics in plants inoculated with G. albida might contribute to the observed potent larvicidal activity. Under field conditions, inoculation of M. tenuiflora with G. albida increases phenolics and larvicidal activity against Ae. aegypti L3 larvae, proving more effective than inoculation with C. etunicatum.

Conclusions

The results herein corroborate mycorrhizal technology for improving biological plant-derived activities, indicating G. albida as the best arbuscular mycorrhizal fungus to improve the larvicidal effects of M. tenuiflora extracts.

Graphical abstract

查看原文本刊更多论文

田间条件下接种丛枝菌根真菌对含含草杀虫活性和酚类化合物的影响

含羞草（mimosa tenuflora，野生）是巴西东北部民间医药中重要的豆科树木，其提取物已对埃及伊蚊幼虫进行了防治试验。这些测试通常使用在自然环境中收集的成年植物的部分。然而，利用丛枝菌根真菌（AMF）接种技术可以成功地生产出tenuflora幼苗。先前的研究报道了接种对tenuflora生长和次生代谢物积累的益处，而关于接种如何影响提取物生物活性的数据仍然有限。本研究探讨了田间接种丛枝菌根真菌提高天冬假单胞菌产量的潜力，重点是提高其提取物对天冬假单胞菌的总酚含量和杀幼虫活性。蚊。结果与其他处理相比，双翅虫侵染后总酚含量升高，幼虫死亡，致死浓度（LC）降低。酚类物质含量为249.87 mg。G−1,48h后死亡比例为68.33%。48 h LC50和LC90值为147µg。mL−1和1301.83µg。mL−1为接种了紫花莲的植物提取物。未接种对照的LC50为800.67µg。mL−1和LC90 8194.26µg。LC50为1179.16µg。mL−1和LC90 3050.32µg。毫升−1。接种弓形虫提取物与未接种对照的幼虫死亡率无显著差异。结果表明，绿足菌接种植株后总酚含量的增加可能是其具有较强的杀虫活性的原因之一。在田间条件下，用绿僵菌接种tenuflora可提高对伊蚊的酚类和杀幼虫活性。埃及伊蚊L3幼虫，证明比接种弓形伊蚊更有效。结论本研究结果证实了菌根技术对提高植物源性生物活性的作用，表明绿僵菌是提高天冬藤提取物杀幼虫效果的最佳菌根真菌。图形抽象

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来源期刊

Beni-Suef University Journal of Basic and Applied Sciences MULTIDISCIPLINARY SCIENCES-

CiteScore

2.60

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0.00%

发文量

期刊介绍： Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.