Study of poly (butylene adipate-co-terephthalate)/beta-cyclodextrin/polyvinyl alcohol, doped Mg2+, coated recycled Fe3O4 particle carriers for enhancing immobilization penicillin G acylase performance

IF 2.2 4区化学 Q3 CHEMISTRY, PHYSICAL

Colloid and Polymer Science Pub Date : 2025-02-25 DOI:10.1007/s00396-025-05391-x

Monier Alhadi Abdelrahman Mohammed, Zhenbin Chen, Xueyan Du, Bin Li, Jinhan Zhang, Tai Kang

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Abstract

In this study, Fe₃O₄ magnetic particles (MPs) were produced from nickel slag using molten oxidation and magnetic separation methods. After that, Fe₃O₄ MPs were coated with a blend of poly (butylene adipate-co-terephthalate)/beta-cyclodextrin/polyvinyl alcohol (PBAT/B-CD/PVA), and Mg²⁺ was doped during this process. Mg²⁺-Fe₃O₄@PBAT/B-CD/PVA MPs were then modified using γ-glycidoxypropyltrimethoxysilane (KH560) to produce Mg²⁺-Fe₃O₄@PBAT/B-CD/PVA-g-KH560 MPs. The immobilized PGA (Mg²⁺-Fe₃O₄@PBAT/B-CD/PVA-g-KH560-PGA MPs) was obtained through covalently bonding PGA under mild circumstances by a click reaction between the amino group of PGA and the epoxy group of KH560. Furthermore, a vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy-energy spectroscopy of dispersive x-rays (SEM–EDS), X-ray photoelectron spectrophotometry (XPS), and inductively coupled plasma mass spectrometry (ICP-MS) were used to characterize the magnetic composite structures. The results showed that the immobilized PGA had values of 31,574 U/g, 94.2%, and 113 mg/g for enzyme activity recovery (EAR), enzyme loading capacity (ELC), and enzyme activity (EA), respectively. Its enzyme activity was maintained at 74% after 12 cycles of usage. The Fe₃O₄@PBAT/B-CD/PVA MPs were prepared similarly to the preparation of Mg²⁺-Fe₃O₄@PBAT/b-CD/PVA MPs except that Mg²⁺ was not doped to it to adopt as control, and after 6 cycles of usage, the immobilized PGA (Fe₃O₄@PBAT/B-CD/PVA-g-KH560-PGA MPs) retained 50% of its original enzyme activity, with EA, EAR, and ELC values of 28,513 U/g, 85.2%, and 107 mg/g, respectively, which were lower than Mg²⁺-Fe₃O₄@PBAT/B-CD/PVA-g-KH560 MPs; this indicate that doped Mg²⁺ had positive impact on immobilized PGA.

Graphical Abstract

Preparation scheme of immobilized PGA (Mg⁺²-Fe₃O₄@PBAT/B-CD/PVA-g-KH560-PGA MPs)

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聚己二酸丁二酯/ -环糊精/聚乙烯醇掺杂Mg2+包被再生Fe3O4颗粒载体提高固定化青霉素G酰化酶性能的研究

以镍渣为原料，采用熔融氧化法和磁选法制备了Fe3O4磁性颗粒。然后，用聚己二酸丁二酯/ β -环糊精/聚乙烯醇（PBAT/B-CD/PVA）共混物包覆Fe3O4 MPs，并在此过程中掺杂Mg2+。然后用γ-缩水氧基丙基三甲氧基硅烷（KH560）修饰Mg2+-Fe3O4@PBAT/B-CD/PVA MPs，得到Mg2+-Fe3O4@PBAT/B-CD/PVA-g-KH560 MPs。通过PGA的氨基与KH560的环氧基之间的咔嗒反应，在温和的条件下通过PGA共价键合得到固定化PGA （Mg2+-Fe3O4@PBAT/B-CD/PVA-g-KH560-PGA MPs）。利用振动样品磁强计（VSM）、傅里叶变换红外光谱（FTIR）、x射线衍射（XRD）、扫描电镜-色散x射线能谱（SEM-EDS）、x射线光电子分光光度（XPS）和电感耦合等离子体质谱（ICP-MS）对磁性复合材料结构进行了表征。结果表明，固定化PGA的酶活回收率（EAR）、酶载量（ELC）和酶活（EA）分别为31574 U/g、94.2%和113 mg/g。12个循环后，其酶活性保持在74%。Fe3O4@PBAT / B-CD / PVA议员准备同样的制备Mg2 + -Fe3O4@PBAT / B-CD / PVA议员除了Mg2 +不掺杂它采取控制,6周期的使用之后,固定化的PGA (Fe3O4@PBAT / B-CD / PVA-g-KH560-PGA MPs)保留50%的原始的酶活性,与EA,耳朵,和复杂形状的28513 U / g值85.2%,和107毫克/克,分别是低于Mg2 + -Fe3O4@PBAT / B-CD / PVA-g-KH560议员;这表明Mg2+的掺杂对固定化PGA有积极的影响。固定化PGA （Mg+2-Fe3O4@PBAT/B-CD/PVA-g-KH560-PGA MPs）制备方案

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来源期刊

Colloid and Polymer Science 化学-高分子科学

CiteScore

4.60

自引率

4.20%

发文量

111

审稿时长

2.2 months

期刊介绍： Colloid and Polymer Science - a leading international journal of longstanding tradition - is devoted to colloid and polymer science and its interdisciplinary interactions. As such, it responds to a demand which has lost none of its actuality as revealed in the trends of contemporary materials science.