Survival of Salmonella Typhimurium, Listeria monocytogenes, and their surrogates on cantaloupes after triple-washing with peroxyacetic acid

IF 6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

LWT - Food Science and Technology Pub Date : 2025-04-25 DOI:10.1016/j.lwt.2025.117821

Md Shafiul Islam Rion , Corey Coe , Joseph Katz , Gary Freshour , Carly Long , Kristen Matak , Annette Freshour , Jacek Jaczynski , Timothy Boltz , Xue Tang , Cangliang Shen

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引用次数: 0

Abstract

The study aimed to evaluate the survival of Salmonella Typhimurium, Enterococcus faecium, Listeria monocytogenes, and Listeria innocua on cantaloupes during storage and after triple-wash exposure (water-water-antimicrobial) with peroxyacetic acid (PAA). Bacteria were spot-inoculated onto cantaloupe surfaces prior to triple-washing for 45 s at each washing step using 0, 27, 54, and 96 ppm of PAA. Spread-plating and a modified most-probable-number (MPN) method were utilized for enumeration of cells. Results showed that D-values (pre-wash) for S. Typhimurium (2.47 h) on cantaloupes were lower (P < 0.05) than E. faecium (28.41 h), and the D-values (pre-wash) of L. monocytogenes (4.50 h) is also lower (P < 0.05) than L. innocua (7.24 h). Though all tested concentrations of PAA resulted in significant (P < 0.05) reduction of bacteria cells compared to the inoculated unwashed control, the greatest (P < 0.05) reduction of S. Typhimurium and E. faecium (4.55 vs 3.23 log₁₀ CFU/cm² and 3.38 vs 2.55 log₁₀ MPN/mL) occurred at 96 ppm. This concentration (96 ppm) also achieved 3–4 log reductions of L. monocytogenes and L. innocua population. Results suggest that E. faecium could be used as surrogate of S. Typhimurium and L. monocytogenes for antimicrobial washing validation studies on cantaloupes.

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过氧乙酸三次洗涤后哈密瓜上鼠伤寒沙门菌、单核增生李斯特菌及其替代物的存活

本研究旨在评估鼠伤寒沙门菌、屎肠球菌、单核增生李斯特菌和无害李斯特菌在哈密瓜储存期间和用过氧乙酸（PAA）三次洗涤（水-水-抗菌）后的存活情况。在使用0、27、54和96 ppm的PAA进行三次洗涤之前，将细菌接种到哈密瓜表面，每一步洗涤45秒。采用铺层法和改进的最可能数法（MPN）进行细胞计数。结果表明，预洗（2.47 h）对哈密瓜鼠伤寒沙门氏菌的d值较低(P <；0.05)高于粪肠杆菌（28.41 h），且单核增生乳杆菌（4.50 h）的d值（预洗）也较低(P <；各组PAA浓度均显著（P < P < 0.05）高于L. innocua （7.24 h）。0.05)，细菌细胞减少量最大(P <；0.05)鼠伤寒沙门氏菌和粪肠杆菌（4.55 vs 3.23 log10 CFU/cm2和3.38 vs 2.55 log10 MPN/mL）在96 ppm时减少。该浓度（96 ppm）也使单核增生乳杆菌和无瘤乳杆菌的种群减少了3-4倍。结果表明，粪肠杆菌可作为鼠伤寒沙门氏菌和单核增生乳杆菌的替代品，用于哈密瓜的抗菌洗涤验证研究。

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来源期刊

LWT - Food Science and Technology 工程技术-食品科技

CiteScore

11.80

自引率

6.70%

发文量

1724

审稿时长

65 days

期刊介绍： LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.