Cutting-edge RNA quantitative detection methods for rapid and on-site analysis

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-04-19 DOI:10.1016/j.trac.2025.118274

Tao Dong , Chenghui Yu , Feng Han , Qi Mao , Weixuan Jing , Zhaochu Yang , Nuno Miguel Matos Pires , José Higino Correia , Qijing Lin , Fei Hu , Honghong Yao , Kui Hu , Tao Xu , Zhuangde Jiang

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Abstract

RNA, a vital biomarker, is crucial for molecular diagnostics, encompassing pathogen detection, gene expression, tumor diagnosis, prognosis, and drug efficacy. Since the COVID-19 outbreak, its role in clinical settings has grown. This review examines established RNA detection methods like qPCR, sequencing, Nanostring, and fluorescence in situ hybridization (FISH), highlighting recent progress and trends. It also focuses on new Point-of-Care Testing (POCT) methods developed over the last five years, categorizing them by the necessity for reverse transcription and nucleic acid amplification. The review emphasizes advances in multiplex detection tailored for POCT, aiming to update researchers and clinicians on the latest RNA detection technologies. Among various RNA detection methods, the amplification-free approach is preferable for POCT applications compared to those requiring reverse transcription and sequence-dependent amplification, as they simplify the detection process by reducing technical complexity while retaining high sensitivity.

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尖端的RNA定量检测方法，用于快速和现场分析

RNA是一种重要的生物标志物，在分子诊断中起着至关重要的作用，包括病原体检测、基因表达、肿瘤诊断、预后和药物疗效。自2019冠状病毒病暴发以来，其在临床环境中的作用越来越大。本文综述了现有的RNA检测方法，如qPCR、测序、纳米链和荧光原位杂交（FISH），重点介绍了最近的进展和趋势。报告还重点介绍了过去五年中开发的新的即时检测（POCT）方法，并根据反转录和核酸扩增的必要性对这些方法进行了分类。这篇综述强调了针对POCT的多重检测技术的进展，旨在为研究人员和临床医生提供最新的RNA检测技术。在各种RNA检测方法中，与需要逆转录和序列依赖扩增的方法相比，无扩增方法更适合POCT应用，因为它们通过降低技术复杂性简化了检测过程，同时保持了高灵敏度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.