Nucleic acid-mediated SERS Biosensors: Signal enhancement strategies and applications

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-04-25 DOI:10.1016/j.bios.2025.117519

Yushi Xie , Jiaqiang Huang , Min Yang , Yangzi Zhang , Xiaobo Zhang , Wentao Xu , Jijuan Cao , Longjiao Zhu

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引用次数: 0

Abstract

Surface Enhanced Raman Spectroscopy (SERS) is a powerful spectroscopic analysis technique applied in various fields due to its high selectivity, ultra-high sensitivity, and non-destructiveness. As natural biological macromolecules, nucleic acids perform a significant role in SERS biosensing. In this review, we first summarize how nucleic acids mediate the signal enhancement of SERS biosensors from three aspects: substrate self-assembly, analyte biorecognition, and molecular amplification. Among them, SERS substrates can be self-assembled by both DNA modification and coordination or electrostatic interactions. In the field of biorecognition, analyte biorecognition based on three nucleic acid recognition elements can enhance SERS signals by regulating the distance of analytes or Raman reporter molecules to the SERS substrate. In addition, nucleic acid-based enzyme and enzyme-free amplification can enhance SERS signals by enlarging the quantity of analytes or its nucleic acid intermediates. Subsequently, multidimensional applications of nucleic acid-mediated SERS signal enhancement in biomedicine, food safety, and environmental monitoring are listed. Finally, the current challenges and future exploration of nucleic acid-mediated SERS signal enhancement are discussed.

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核酸介导的SERS生物传感器：信号增强策略及应用

表面增强拉曼光谱（SERS）以其高选择性、超高灵敏度、无损等优点，被广泛应用于各个领域。核酸作为天然的生物大分子，在SERS生物传感中发挥着重要的作用。本文首先从底物自组装、被分析物生物识别和分子扩增三个方面综述了核酸介导SERS生物传感器信号增强的机制。其中，SERS底物可以通过DNA修饰和配位或静电相互作用进行自组装。在生物识别领域，基于三种核酸识别元件的被分析物生物识别可以通过调节被分析物或拉曼报告分子到SERS底物的距离来增强SERS信号。此外，基于核酸的酶和无酶扩增可以通过增加分析物或其核酸中间体的数量来增强SERS信号。随后列举了核酸介导的SERS信号增强在生物医学、食品安全、环境监测等方面的多维应用。最后，讨论了核酸介导的SERS信号增强目前面临的挑战和未来的探索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.