The gene characteristics and adaptive evolution of the tumor necrosis factor superfamily (TNFSF) in miiuy croaker, Miichthys miiuy

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-04-25 DOI:10.1016/j.fsi.2025.110369

Xiaoqin Su , Yanqiu Cui , Hanfu Gong , Tianjun Xu , Yuena Sun

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引用次数: 0

Abstract

The tumor necrosis factor superfamily (TNFSF) is crucial in regulating immune responses, with its members mediating various biological functions through key signaling pathways. However, the gene characteristics of this family and their comparative and evolutionary analysis across species remain limited. In this study, 12 TNFSF genes were identified in the genome-wide of miiuy croaker. Analyses were conducted on evolutionary relationships, conserved motifs, gene duplication, and selection pressure. Conserved motif analyses revealed that the C-terminal motifs of vertebrate TNFSF proteins were more conserved than the N-terminus. Sequence alignment and conservation analysis identified an unrecognized helix structure within the TNF homology domain, which exhibited structural conservation among vertebrates. Synteny and selection pressure analyses indicated that the TNFSF in miiuy croaker exhibited tandem and segmental duplication events. Evolutionary selection pressures may contributed to the functional differentiation of this family. These findings could enhance the understanding of TNFSF gene characteristics and evolutionary relationships, and provide new insights for studying immune-related TNFSF genes.

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黑花鱼肿瘤坏死因子超家族（TNFSF）的基因特征及适应性进化

肿瘤坏死因子超家族（tumor necrosis factor superfamily， TNFSF）在调节免疫应答中起着至关重要的作用，其成员通过关键的信号通路介导各种生物功能。然而，该科的基因特征及其跨物种的比较和进化分析仍然有限。本研究在马家鱼全基因组中鉴定了12个TNFSF基因。分析了进化关系、保守基序、基因复制和选择压力。保守基序分析表明，脊椎动物TNFSF蛋白的c端基序比n端基序更为保守。序列比对和保守分析在TNF同源结构域中发现了一个未被识别的螺旋结构，该结构在脊椎动物中表现出结构保守性。同步性分析和选择压力分析表明，小花鱼的TNFSF存在串联复制和片段复制。进化选择压力可能促成了这个家族的功能分化。这些发现有助于加深对TNFSF基因特征和进化关系的认识，并为研究免疫相关的TNFSF基因提供新的见解。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.