Efficient nitrogen removal by the aerobic denitrifying bacterium Pseudomonas stutzeri RAS-L11 under triple stresses of high alkalinity, high salinity, and tetracycline: From performance to mechanism

Abstract

Efficient aerobic denitrification bacteria are rarely reported under triple stresses of high alkalinity, high salinity, and tetracycline. Here, strain Pseudomonas stutzeri RAS-L11 was isolated, under the optimal reaction conditions of C/N = 6, sodium acetate as carbon source, and pH 7.0–11.0. Moreover, RAS-L11 showed perfect nitrogen removal performance under dual and triple stresses. Specifically, the mean removal efficiency of total dissolved nitrogen for different medium (nitrate, nitrite, ammonia, nitrate and ammonia, and nitrite and ammonia) reached 92.35 %, 66.85 %, 71.33 %, 89.42 %, and 68.76 % under triple stresses. Nitrogen balance results indicated that biomass nitrogen accounted for a small percentage (4.48 % to 20.79 %). Furthermore, the nitrogen metabolism pathways and tetracycline, salinity, and alkaline tolerance-associated genes were also confirmed. Strain RAS-L11 achieved 42.67–70.72 % NO₃⁻-N and 83.72–88.53 % NH₄⁺-N removal efficiencies in both sterilized and actual systems treating pharmaceutical wastewater. Our characterization of the RAS-L11 provides a reference for nitrogen removal of pharmaceutical wastewater.