Encapsulation of Lactiplantibacillus plantarum in W/O/W emulsion stabilized with whey protein isolate and konjac glucomannan complex

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2025-04-25 DOI:10.1016/j.carbpol.2025.123661

Jiahui Guo , Cong Xu , XinYu Neng , Jiamin Zhang , Xuefei Wu , Xuan Liu , Yongchen Liu , Zhongmei Tan , Zhanmei Jiang , Juncai Hou

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引用次数: 0

Abstract

This experiment investigated the encapsulation of Lactiplantibacillus plantarum 1.0317 in a water-in-oil-in-water (W/O/W) emulsion to significantly enhance the activity of Lactiplantibacillus plantarum 1.0317 under storage conditions, during pasteurization, and in simulated in vitro gastrointestinal digestion environments. Lactiplantibacillus plantarum 1.0317 was encapsulated in emulsion using whey protein isolate (WPI) alone or non-covalent complex of whey protein isolate and konjac glucomannan (KGM). Compared with the WPI-stabilized emulsion, the WPI-KGM stabilized emulsion had smaller particle size, better rheological properties, higher embedding efficiency and markedly improved the vitality of Lactiplantibacillus plantarum 1.0317 after storage, pasteurization, low pH treatment, high concentration of bile salt treatment and simulated gastrointestinal digestion. As the mass ratio of WPI-KGM composite was 3:1, the highest embedding efficiency of W/O/W emulsion was 89.76 %. Moreover, compared with the free Lactiplantibacillus plantarum 1.0317 and its other encapsulated emulsion, when the WPI-KGM complex mass ratio is 3:1, the W/O/W emulsion containing Lactiplantibacillus plantarum 1.0317 has the highest activity of probiotics after 28 days of storage, pasteurization and simulated gastrointestinal digestion, which are 97.03 %, 74.15 % and 70.07 %, respectively. This study offers a novel idea for the emulsion system used in probiotic delivery based on WPI-KGM.

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乳清分离蛋白和魔芋葡甘露聚糖络合物稳定W/O/W乳剂中植物乳杆菌的包封

本实验研究了将植物乳杆菌1.0317包封在水包油（W/O/W）乳状液中，在储存条件、巴氏灭菌条件和模拟体外胃肠消化环境下，显著提高植物乳杆菌1.0317的活性。采用乳清分离蛋白（WPI）或乳清分离蛋白与魔芋葡甘露聚糖（KGM）的非共价配合物包封植物乳杆菌1.0317。与WPI-KGM稳定乳剂相比，WPI-KGM稳定乳剂经储存、巴氏灭菌、低pH处理、高浓度胆盐处理和模拟胃肠道消化后，粒径更小、流变性能更好、包埋效率更高，显著提高了植物乳杆菌1.0317的活力。当WPI-KGM复合材料的质量比为3:1时，W/O/W乳液的最高包埋率为89.76%。此外，与游离植物乳杆菌1.0317及其包封乳相比，当WPI-KGM复合物质量比为3:1时，含植物乳杆菌1.0317的W/O/W乳经28 d储存、巴氏灭菌和模拟胃肠道消化后的益生菌活性最高，分别为97.03%、74.15%和70.07%。本研究为基于WPI-KGM的益生菌乳剂体系提供了一种新的思路。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.