Synthesis of fluorescein modified carbon quantum dots and application of dual emission ratio fluorescent probes for highly selective detection of chlorothalonil

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-21 DOI:10.1016/j.saa.2025.126278

Qing Li , Jianghua Wang , Zikang Zhang , Yiluo Xu , Yingqiang Fu

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引用次数: 0

Abstract

Chlorothalonil (CHT) is a class 2B carcinogen that is capable of remaining in the environment for long periods, has an obvious bioconcentration phenomenon, and may pollute groundwater, thus it is essential to construct a simple detection method for chlorothalonil. However, common single emission fluorescent probes or dual emission fluorescent probes constructed from multi-component mixtures have low selectivity and anti-interference ability. In this work, a simple two-step hydrothermal method was used to synthesize fluorescein-modified carbon quantum dots (F-CQDs), and a dual-emission fluorescent probe was constructed to detect chlorothalonil concentration. The study found that in the presence of a certain amount of Fe²⁺, the fluorescence intensity of the fluorescence emission peaks of F-CQDs located at 435 nm and 508 nm gradually decreased with the increase of CHT concentration within 2 min. This fluorescent probe has specific recognition for CHT and shows a significant linear relationship in the range of 0.1664–3.0695 μM, with a detection limit as low as 8.6 × 10⁻³ μM. The probe was successfully applied to the detection of CHT residual content in environmental water with a satisfactory average recovery, and the present study provides a new method for the preparation of dual-emission fluorescent probes and the detection of organic pesticides and shows great potential in environmental monitoring.

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荧光素修饰碳量子点的合成及双发射比荧光探针高选择性检测百菌清的应用

百菌清（Chlorothalonil， CHT）是2B类致癌物，可在环境中长期残留，具有明显的生物富集现象，并可能污染地下水，因此建立一种简便的百菌清检测方法十分必要。然而，普通的单发射荧光探针或由多组分混合物构成的双发射荧光探针的选择性和抗干扰能力较低。本文采用简单的两步水热法合成荧光素修饰的碳量子点（F-CQDs），并构建了双发射荧光探针来检测百菌清浓度。研究发现，在一定量的Fe2+存在下，在2 min内，位于435 nm和508 nm处的F-CQDs荧光发射峰的荧光强度随着CHT浓度的增加而逐渐降低。该荧光探针对CHT具有特异性识别，在0.1664 ~ 3.0695 μM范围内呈显著线性关系，检出限低至8.6 × 10−3 μM。该探针成功应用于环境水中CHT残留量的检测，平均回收率令人满意，为双发射荧光探针的制备和有机农药的检测提供了一种新的方法，在环境监测中具有很大的潜力。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.