Electrochemical process modulating colorimetry for dual-mode reliable detection of patulin based on target-induced DNA walker-triggered RCA-CHA cascade amplification

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-04-28 DOI:10.1016/j.snb.2025.137881

Chenchen Jin , Yu Ya , Yunzhi Ma , Qingnian Wu , Jun Yan , Ke-Jing Huang , Xuecai Tan , Yun Duan

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Abstract

Patulin (PAT) is a toxic fungal metabolite widely present in nature, posing a significant threat to human and animal health. Herein, a self-powered sensing platform is developed for electrochemical/colorimetric detection of PAT, which leverages a target-induced DNA Walker-triggered rolling circle amplification (RCA) and catalytic hairpin assembly (CHA) cascade reaction, along with the synergistic catalysis of nanoenzymes and biological enzymes. In the detection system, the presence of PAT leads to the specific binding of the aptamer to activate the DNA Walker, which subsequently triggers the RCA-CHA cascade reaction, and then adsorbs abundant methylene blue to enhance the output signal. Under optimized conditions, the linear detection range of this method is 10⁻¹³-10⁻⁵ mg/mL, with detection limits of 2.8 × 10⁻¹⁴ mg/mL (electrochemical) and 1.8 × 10⁻¹⁴ mg/mL (colorimetric). The method is applied to detect PAT in banana samples with recoveries of 80.0–106.0 %, which demonstrates its high accuracy and significant potential for practical application.

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基于靶诱导DNA步行者触发RCA-CHA级联扩增的电化学过程调制比色法双模可靠检测展霉素

棒曲霉素（PAT）是一种广泛存在于自然界的有毒真菌代谢物，对人类和动物的健康构成重大威胁。本文开发了一种用于电化学/比色检测PAT的自供电传感平台，该平台利用了靶诱导的DNA walker触发的滚环扩增（RCA）和催化发夹组装（CHA）级联反应，以及纳米酶和生物酶的协同催化。在检测系统中，PAT的存在导致适体特异性结合激活DNA Walker，进而触发RCA-CHA级联反应，然后吸附大量亚甲基蓝增强输出信号。在优化条件下，该方法的线性检测范围为10-13-10-5 mg/mL，检出限分别为2.8×10-14 mg/mL（电化学）和1.8×10-14 mg/mL（比色）。该方法用于香蕉样品中PAT的检测，回收率为80.0 ~ 106.0%，具有较高的准确度和较好的实际应用潜力。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.