Crotalaria juncea reduces larval survival and adult fecundity of Diabrotica speciosa

IF 4.1 1区农林科学 Q1 ENTOMOLOGY

Journal of Pest Science Pub Date : 2025-04-28 DOI:10.1007/s10340-025-01890-6

Caroline Rech, Leandro do Prado Ribeiro, José Maurício Simões Bento, Eduardo José Crevelin, Cristiano André Pott, Cristiane Nardi

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引用次数: 0

Abstract

In this study, we hypothesized that Crotalaria juncea (L., Fabaceae), particularly due to its content of the pyrrolizidine alkaloid monocrotaline, would affect the development, fecundity, and longevity of Diabrotica speciosa (Germar) Coleoptera: Chrysomelidae). We initially assessed the effects of C. juncea plants and their various parts (leaves, stems, and roots) on the insect. Newly hatched larvae were inoculated in containers with (i) popcorn plants (Zea mays L. var everta); (ii) C. juncea plants; (iii) popcorn plants associated with C. juncea; and (iv) popcorn plants with portions of leaves, stems, and roots (mulch) of C. juncea (25 ± 2 °C, 60 ± 10% RH, and a 14-h photoperiod). The larvae were kept in these conditions until adult emergence, after which the adults were provided with common bean leaflets. The presence of C. juncea during the larval stage influenced the biological parameters of D. speciosa. We observed 100% larval mortality when exposed to isolated C. juncea plants, and a decrease in immature survival and adult longevity when C. juncea was associated with popcorn. In a subsequent trial, we investigated the effects of crude organic extracts of C. juncea on the same biological parameters. Newly hatched larvae were placed on popcorn plants with roots treated with extracts from different parts of C. juncea (aerial parts, roots, and entire plants) and using various solvents (hexane, dichloromethane, and methanol) as extractors. Plants with roots treated with root extracts of C. juncea exhibited lower survival rates during the immature stage, as well as reduced fecundity and egg viability. This indicates that allelochemicals from C. juncea have a detrimental impact on the development, fecundity, and viability of D. speciosa eggs. Chemical analysis of the C. juncea extracts revealed that monocrotaline, while present in various plant parts, is not the sole component responsible for the observed effects on the insects.

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芥菜瓣虫降低了Diabrotica speciosa的幼虫存活率和成虫繁殖力

在本研究中，我们假设Crotalaria juncea （L., Fabaceae)，特别是由于其吡啶类生物碱monocrotaline的含量，会影响Diabrotica speciosa（德国）鞘翅目：金鸡科）的发育、繁殖力和寿命。我们初步评估了芥菜植物及其各部分（叶、茎和根）对昆虫的影响。将新孵化的幼虫接种在装有(1)玉米花（Zea mays L. var everta）的容器中；（ii）芥菜；（iii）与芥菜有关的玉米花植物；（iv）以甘薯部分叶、茎和根（地膜）为材料的爆米花植株（25±2℃，60±10% RH， 14 h光周期）。幼虫在这些条件下饲养，直到成虫羽化，之后给成虫提供普通豆叶。幼虫期芥菜菌的存在对褐家蚕的生物学参数有影响。我们观察到，当暴露于分离的芥菜植株时，幼虫死亡率为100%，而当与爆米花接触时，芥菜的未成熟存活率和成虫寿命均有所下降。在随后的试验中，我们研究了芥子草有机粗提物对相同生物学参数的影响。将新孵化的幼虫置于玉米花植株上，用不同部位（地上部分、根部和整株）的提取物和不同溶剂（己烷、二氯甲烷和甲醇）作为萃取剂处理玉米花植株的根。用芥菜根提取物处理过的植株在未成熟期的成活率较低，繁殖力和卵活力也降低。这表明芥菜的化感物质对芥菜卵的发育、繁殖力和生存能力有不利影响。对芥菜提取物的化学分析表明，虽然存在于植物的不同部位，但单苦杏仁碱并不是对昆虫产生影响的唯一成分。

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来源期刊

Journal of Pest Science 生物-昆虫学

CiteScore

10.40

自引率

8.30%

发文量

114

审稿时长

6-12 weeks

期刊介绍： Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.