Reversing inhibition to promotion in phenol-ammonium metabolism via algal-microbial fuel cell: mechanisms of phenol-ammonium interaction and synergistic removal

Abstract

Addressing the challenge of metabolic inhibition between phenol and ammonium in coal gasification wastewater (CGW), this study introduced a novel algal-microbial fuel cell (AMFC). It combined the advantages of electroactive bacteria and Synechocystis to achieve synergistic metabolism, establishing a cooperative mechanism for pollutant separation and enhanced transformation to achieve the mutual promotion of phenol and ammonium removal. Remarkably, raising phenol to 1500 mg COD/L boosted ammonium removal by 31.51% in AMFC, due to a consistently higher potential difference than the control, which enhanced extracellular electron transfer (EET) via conductive nanowire and drove ammonium migration. Similarly, elevating ammonium concentration to 150 mg/L resulted in an 11.79% increase in phenol removal efficiency, driven by superior solution conductivity and EET, as well as more electron acceptors (oxygen) from the algal cathode. This system challenged the conventional understanding of the antagonistic relationship between phenol and ammonium. Under high phenol conditions, the electroactive bacteria Clostridium sensu stricto 1 and Acinetobacter, Perlucidibaca formed a synergistic metabolic network, whereas Zoogloea, Ideonella, and other phenol-degrading bacteria were significantly enriched in high ammonium environments. The AMFC represented a breakthrough in reversing the metabolic inhibition between phenol and ammonium, providing a novel and energy-efficient strategy for treating complex industrial wastewater.