Doxorubicin and NFL-TBS.40-63 peptide loaded gold nanoparticles as a multimodal therapy of glioblastoma

IF 5.5 3区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nanoscale Research Letters Pub Date : 2025-04-28 DOI:10.1186/s11671-025-04249-z

Myriam El Moutaoukil, Maria Grazia Lolli, Stefania D’Amone, Memona Khan, Roberta Grillo, Joel Eyer, Maddalena Grieco, Ornella Ursini, Jolanda Spadavecchia, Barbara Cortese

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引用次数: 0

Abstract

Conventional treatments for glioblastoma (GBM) are hindered by systemic toxicity, limited blood–brain barrier penetration, and therapeutic resistance. To address these challenges, we developed dual-functionalized gold nanoparticles (AuNPs) conjugated with a biotinylated NFL-TBS.40-63 peptide and the chemotherapeutic agent doxorubicin. This platform integrates targeted delivery and therapeutic action to enhance efficacy while minimising off-target effects. Our findings reveal superior cellular uptake, dose- and time-dependent cytotoxicity, and apoptosis induction in GBM cells compared to mono-functionalized counterparts. Furthermore, pH-sensitive drug release profiles underscore the system’s potential to exploit the tumour microenvironment’s acidic conditions for precise drug delivery. Comprehensive characterisation confirmed the stability, biocompatibility, and functional efficacy of the dual-functionalized AuNPs. This study highlights the promise of these nanoconjugates as a multimodal approach to GBM therapy, paving the way for further translational research in nanomedicine.

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阿霉素与NFL-TBS。40-63肽负载金纳米颗粒作为胶质母细胞瘤的多模式治疗

胶质母细胞瘤（GBM）的常规治疗受到全身毒性、有限的血脑屏障穿透和治疗耐药性的阻碍。为了解决这些挑战，我们开发了双功能化金纳米颗粒（AuNPs）与生物素化的fl - tbs结合。40-63肽和化疗药物阿霉素。该平台整合了靶向递送和治疗行动，以提高疗效，同时最大限度地减少脱靶效应。我们的研究结果显示，与单功能化的细胞相比，GBM细胞具有更强的细胞摄取、剂量和时间依赖性的细胞毒性和诱导凋亡的能力。此外，ph敏感的药物释放谱强调了该系统利用肿瘤微环境的酸性条件进行精确药物递送的潜力。综合表征证实了双功能化aunp的稳定性、生物相容性和功能功效。这项研究强调了这些纳米缀合物作为GBM治疗的多模态方法的前景，为纳米医学的进一步转化研究铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanoscale Research Letters 工程技术-材料科学：综合

CiteScore

11.30

自引率

0.00%

发文量

110

审稿时长

48 days

期刊介绍： Nanoscale Research Letters (NRL) provides an interdisciplinary forum for communication of scientific and technological advances in the creation and use of objects at the nanometer scale. NRL is the first nanotechnology journal from a major publisher to be published with Open Access.