Morphological and molecular characterization of a pure isolate of Eimeria lata, identified for the first time in domestic chickens in Brazil

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-04-25 DOI:10.1016/j.vprsr.2025.101265

Bruno Ferraz Itoyama , Bruno Pereira Berto , Guilherme Zaratin Dumalakas , Brayan Kurahara , Eduardo Anzai Furlaneti , Marcelo Vasconcelos Meireles

{"title":"Morphological and molecular characterization of a pure isolate of Eimeria lata, identified for the first time in domestic chickens in Brazil","authors":"Bruno Ferraz Itoyama , Bruno Pereira Berto , Guilherme Zaratin Dumalakas , Brayan Kurahara , Eduardo Anzai Furlaneti , Marcelo Vasconcelos Meireles","doi":"10.1016/j.vprsr.2025.101265","DOIUrl":null,"url":null,"abstract":"<div><div>Coccidiosis of domestic chickens is caused by seven Eimeria species, in addition to three recently proposed species: Eimeria lata, Eimeria nagambie, and Eimeria zaria. This study reports the first identification of E. lata in domestic chickens in Brazil, the successful establishment of a pure E. lata isolate, and its morphological and molecular analyses. Fecal samples were collected from six extensive production systems in the state of São Paulo, Brazil, and screened for the 10 Eimeria species of domestic chickens through species-specific PCRs. To obtain E. lata oocysts, a mixture of oocysts from various Eimeria species was sequentially administered to commercial broiler chickens vaccinated against coccidiosis. Subsequently, a pure isolate of E. lata was obtained by micromanipulation and propagated in chickens. Eimeria lata oocysts were further subjected to morphological and molecular characterization. In conclusion, these findings constitute the first documentation of E. lata in domestic chickens in Brazil. The Brazilian E. lata isolate, designated BR-AMC, exhibited molecular and morphological characteristics similar to those of E. lata isolates from other countries.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"61 ","pages":"Article 101265"},"PeriodicalIF":1.4000,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology, regional studies and reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405939025000735","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Coccidiosis of domestic chickens is caused by seven Eimeria species, in addition to three recently proposed species: Eimeria lata, Eimeria nagambie, and Eimeria zaria. This study reports the first identification of E. lata in domestic chickens in Brazil, the successful establishment of a pure E. lata isolate, and its morphological and molecular analyses. Fecal samples were collected from six extensive production systems in the state of São Paulo, Brazil, and screened for the 10 Eimeria species of domestic chickens through species-specific PCRs. To obtain E. lata oocysts, a mixture of oocysts from various Eimeria species was sequentially administered to commercial broiler chickens vaccinated against coccidiosis. Subsequently, a pure isolate of E. lata was obtained by micromanipulation and propagated in chickens. Eimeria lata oocysts were further subjected to morphological and molecular characterization. In conclusion, these findings constitute the first documentation of E. lata in domestic chickens in Brazil. The Brazilian E. lata isolate, designated BR-AMC, exhibited molecular and morphological characteristics similar to those of E. lata isolates from other countries.

Abstract Image

查看原文本刊更多论文

首次在巴西家鸡中鉴定的俄美耳球虫纯分离株的形态和分子特征

家鸡的球虫病是由七种艾美球虫引起的，除了最近提出的三种艾美球虫：lata艾美球虫、nagambie艾美球虫和zaria艾美球虫。本研究首次在巴西家鸡中鉴定出拉塔埃氏菌，成功建立了拉塔埃氏菌纯分离株，并对其进行了形态和分子分析。从巴西圣保罗州的6个大规模生产系统收集粪便样本，并通过物种特异性pcr筛选家鸡的10种艾美尔球虫。为了获得拉塔伊夫虫卵囊，将不同艾美耳球虫种的卵囊混合物依次饲喂于接种球虫病疫苗的商品肉鸡。随后，通过显微操作获得了lata的纯分离株，并在鸡中进行了繁殖。进一步对俄美球虫卵囊进行形态和分子表征。综上所述，这些发现构成了巴西家鸡中巴西埃氏绦虫的首次文献记录。巴西的巴西埃米塔分离物BR-AMC与其他国家的埃米塔分离物具有相似的分子和形态特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).