Tissue-specific kinetics of glycerol kinase and its expression pattern in mouse: a comparative analysis

IF 1 Q4 GENETICS & HEREDITY
Riva M. Rani, Basukshisha Wanniang, Bidyadhar Das
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Abstract

Glycerol kinase (GK) catalyzes the transfer of a phosphate group from ATP to glycerol. GK is the key enzyme for utilizing glycerol as a carbon and energy source. The functionality of GK displays distinctive characteristics based on the tissue type, reflecting the distinct glycerol metabolic pathways in different tissues. Enzyme kinetics and western blot analysis were performed using different mouse tissues to determine the activity and protein expression of GK. The highest specific activities of GK were observed in muscle and heart tissues, with 1.433 U/mg protein and 1.451 U/mg protein, respectively. The enzyme exhibited optimal activity at pH 7.5 and showed consistency in various buffers. The highest Vmax for ATP was in muscle and heart tissues (3.075 U/mg protein and 2.743 U/mg protein), with the highest Km for ATP in muscle and testis (0.529 mM and 0.582 mM). Additionally, for glycerol, the highest Vmax was also in muscle and heart tissues (2.970 U/mg protein and 3.220 U/mg protein), with the highest Km in these tissues being 6.496 μM and 7.142 μM, respectively. Western blot analysis indicated that GK is expressed in all tissues analyzed. This study revealed the tissue-specific functionality of GK in various mouse tissues, emphasising its crucial role in glycerol metabolism. This study also provided basic outlines of GK expression patterns in various tissues of mice and may provide important information for the regulation of GK for its critical role in type 2 diabetes mellitus.

Abstract Image

甘油激酶的组织特异性动力学及其在小鼠中的表达模式:比较分析
甘油激酶(GK)催化磷酸基团从ATP向甘油的转移。GK是利用甘油作为碳和能量来源的关键酶。GK的功能根据组织类型表现出不同的特征,反映了不同组织中不同的甘油代谢途径。采用不同小鼠组织进行酶动力学和western blot分析,测定GK的活性和蛋白表达。GK在肌肉和心脏组织的比活性最高,分别为1.433 U/mg蛋白和1.451 U/mg蛋白。该酶在pH为7.5时表现出最佳活性,在不同缓冲液中表现出一致性。肌肉和心脏组织ATP Vmax最高(分别为3.075 U/mg蛋白和2.743 U/mg蛋白),肌肉和睾丸组织ATP Km最高(分别为0.529 mM和0.582 mM)。甘油的Vmax在肌肉和心脏组织中最高(分别为2.970 U/mg蛋白和3.220 U/mg蛋白),Km在肌肉和心脏组织中最高,分别为6.496 μM和7.142 μM。Western blot分析显示GK在所有组织中均有表达。这项研究揭示了GK在各种小鼠组织中的组织特异性功能,强调了它在甘油代谢中的关键作用。该研究还提供了GK在小鼠各组织中的表达模式的基本轮廓,并可能为GK在2型糖尿病中的关键作用的调控提供重要信息。
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来源期刊
Gene Reports
Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.30
自引率
7.70%
发文量
246
审稿时长
49 days
期刊介绍: Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.
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