A fluorescent probe for progressive tracking glyoxal and sulfite and its application in food analysis and biological imaging

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-25 DOI:10.1016/j.saa.2025.126290

Yuanyuan Wu , Chang Liu , Huirong Yao , Song He , Liancheng Zhao , Xianshun Zeng

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Abstract

In this work, a mitochondrial-targeted fluorescent probe (AATC) for the progressive detection of glyoxal and SO₃²⁻ via the formation of a dihydroquinoxaline derivative with glyoxal was developed. The probe exhibited a robust “turn-on” fluorescence response toward glyoxal with high selectivity and sensitivity (0.25 μM) in aqueous solution, and showed potential applications in real samples with high recoveries ranging from 98.12 % to 100.88 %. Furthermore, the probe can monitor both endogenous and exogenous glyoxal, as well as dynamic fluctuations in glyoxal levels during glycolysis and carbonyl stress processes stimulated by acrolein. Importantly, through a red-shifted fluorescence decrease change elicited by the addition reaction on the imine bond of the formed dihydroquinoxaline derivative, the product of the probe with glyoxal can serve as a secondary sensor for sulfite detection, demonstrating effective monitoring capabilities in both aqueous environments and cellular systems.

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一种递进跟踪乙二醛和亚硫酸盐的荧光探针及其在食品分析和生物成像中的应用

在这项工作中，开发了一种线粒体靶向荧光探针（AATC），通过与乙二醛形成二氢喹啉衍生物来逐步检测乙二醛和SO32 -。该探针在水溶液中对乙二醛具有高选择性和高灵敏度（0.25 μM）的“开启”荧光响应，在实际样品中具有98.12% ~ 100.88%的高回收率。此外，该探针可以监测内源性和外源性乙二醛，以及丙烯醛刺激的糖酵解和羰基应激过程中乙二醛水平的动态波动。重要的是，通过形成的二氢喹啉衍生物的亚胺键加成反应引起的红移荧光减少变化，探针与乙二醛的产物可以作为亚硫酸盐检测的二次传感器，在水环境和细胞系统中都显示出有效的监测能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.