{"title":"Correlation of miRNA-124-3p and SP1 expression levels in spinal cord injury","authors":"Maryam Zand , Mehdi Sadegh , Behzad Khansarinejad , Mahdieh Mondanizadeh","doi":"10.1016/j.genrep.2025.102231","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Spinal cord injury (SCI) is a serious traumatic event affecting the central nervous system, leading to severe mobility and functional impairments. MicroRNAs, particularly miR-124, play a crucial role in neurogenesis and neuronal protection in this condition. Concurrently, Specific Protein 1 (SP1) is involved in various cellular processes, including cell growth and apoptosis. This study investigates the relationship between miR-124-3p and SP1 expression levels in a rat model of spinal cord injury.</div></div><div><h3>Methods</h3><div>An experimental study was conducted using 72 male Wistar rats, divided into three groups: SCI, sham, and control. A standardized SCI model was established through a mechanical compression method at the T9-T10 spinal region. Motor function was assessed using the Basso, Beattie, and Bresnahan (BBB) scoring system at four-time points: 1 h, 1 day, 3 days, and 7 days post-injury. Histological examination was performed via Nissl staining, while RT-qPCR was used to quantify miR-124-3p and SP1 expression levels in spinal cord tissues.</div></div><div><h3>Results</h3><div>The BBB scores indicated a significant decline in motor function across all time points post-SCI. Histological analysis confirmed successful injury induction. Notably, miR-124-3p expression decreased significantly in the SCI group compared to controls, whereas SP1 levels increased significantly following injury.</div></div><div><h3>Conclusions</h3><div>This study demonstrates a marked reduction of miR-124-3p alongside an elevation of SP1 levels in response to SCI. These findings suggest a complex interplay between these molecules that may influence neuronal response and recovery mechanisms post-SCI. Understanding this relationship could provide insights into potential therapeutic targets for enhancing recovery following SCIs.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102231"},"PeriodicalIF":1.0000,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452014425001049","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
Background
Spinal cord injury (SCI) is a serious traumatic event affecting the central nervous system, leading to severe mobility and functional impairments. MicroRNAs, particularly miR-124, play a crucial role in neurogenesis and neuronal protection in this condition. Concurrently, Specific Protein 1 (SP1) is involved in various cellular processes, including cell growth and apoptosis. This study investigates the relationship between miR-124-3p and SP1 expression levels in a rat model of spinal cord injury.
Methods
An experimental study was conducted using 72 male Wistar rats, divided into three groups: SCI, sham, and control. A standardized SCI model was established through a mechanical compression method at the T9-T10 spinal region. Motor function was assessed using the Basso, Beattie, and Bresnahan (BBB) scoring system at four-time points: 1 h, 1 day, 3 days, and 7 days post-injury. Histological examination was performed via Nissl staining, while RT-qPCR was used to quantify miR-124-3p and SP1 expression levels in spinal cord tissues.
Results
The BBB scores indicated a significant decline in motor function across all time points post-SCI. Histological analysis confirmed successful injury induction. Notably, miR-124-3p expression decreased significantly in the SCI group compared to controls, whereas SP1 levels increased significantly following injury.
Conclusions
This study demonstrates a marked reduction of miR-124-3p alongside an elevation of SP1 levels in response to SCI. These findings suggest a complex interplay between these molecules that may influence neuronal response and recovery mechanisms post-SCI. Understanding this relationship could provide insights into potential therapeutic targets for enhancing recovery following SCIs.
Gene ReportsBiochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.30
自引率
7.70%
发文量
246
审稿时长
49 days
期刊介绍:
Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.