Transcriptome and carotenogenic genes analyse unlocked β-carotene and astaxanthin overproduction in Coelastrella saipanensis mediating dairy and fish wastewaters remediation

IF 6.3 2区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of water process engineering Pub Date : 2025-04-28 DOI:10.1016/j.jwpe.2025.107820

Nayana Karicheri, Arunkumar Kulanthaiyesu

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Abstract

This present study, for the first time, demonstrated a comprehensive evaluation of Coelastrella saipanensis for sustainable and enhanced production of high-value carotenoids—β-carotene and astaxanthin under nutrient-rich dairy and fish wastewater conditions. The novelty of this work lies in integrating pigment profiling, antioxidant and cytotoxic assessments, and transcriptomic analyses to unravel the species' dual potential in both biorefinery applications and wastewater remediation. Pigments were successfully separated by HPTLC, and LC-MS analysis confirmed the presence of β-carotene and astaxanthin in C. saipanensis, with characteristic peaks at m/z 537.5048 and 597.4106, respectively, corresponding to standard references. Notably, carotenoid accumulation increased significantly by 28.81 % and 13.56 % in dairy and fish wastewater, respectively. The pigment extracts exhibited strong antioxidant activity, with inhibition levels reaching 39.27 %, 43.08 %, and 46.85 % at 60 μg for control, β-carotene, and astaxanthin, respectively. Cytotoxicity assays against MCF-7 cells revealed IC₅₀ values of 118.85, 144.70, and 94.38 μg/mL for the control pigment, β-carotene, and astaxanthin, respectively, highlighting their therapeutic potential. Furthermore, the extracts demonstrated broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria. A key innovation in this study is the de novo transcriptome analysis of C. saipanensis cultivated in dairy wastewater, which elucidated the molecular mechanisms underlying enhanced carotenoid biosynthesis. RT-PCR validation revealed the differential upregulation of key genes involved in carotenoid pathways, indicating significant metabolic shifts triggered by waste-derived nutrients. This multi-dimensional approach not only establishes C. saipanensis as a robust platform for carotenoid production but also underscores its applicability in environmental biotechnology for efficient wastewater valorization.

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转录组和胡萝卜素基因分析了解锁后的β-胡萝卜素和虾青素过量产生在塞班Coelastrella saipanensis介导乳制品和鱼类废水修复中的作用

本研究首次综合评价了在营养丰富的乳制品和鱼类废水条件下，塞班Coelastrella saipanensis可持续和提高高价值类胡萝卜素- β-胡萝卜素和虾青素的产量。这项工作的新颖之处在于整合了色素分析、抗氧化和细胞毒性评估以及转录组学分析，以揭示该物种在生物炼制应用和废水修复中的双重潜力。通过HPTLC成功分离色素，LC-MS分析证实塞班草中存在β-胡萝卜素和虾青素，特征峰分别为m/z 537.5048和597.4106，对应标准参考文献。乳业废水和渔业废水中类胡萝卜素积累量分别显著增加28.81%和13.56%。在60 μg浓度下，对照、β-胡萝卜素和虾青素提取物的抗氧化活性分别达到39.27%、43.08%和46.85%。针对MCF-7细胞的细胞毒性试验显示，对照色素、β-胡萝卜素和虾青素的IC₅0值分别为118.85、144.70和94.38 μg/mL，突出了它们的治疗潜力。此外，提取物对革兰氏阳性菌和革兰氏阴性菌均具有广谱抗菌活性。本研究的一个关键创新是对奶牛废水中培养的C. saipanensis的从头转录组分析，该分析阐明了增强类胡萝卜素生物合成的分子机制。RT-PCR验证揭示了参与类胡萝卜素途径的关键基因的差异上调，表明废物来源的营养物质引发了显著的代谢变化。这种多维方法不仅确立了C. saipanensis作为类胡萝卜素生产的强大平台，而且强调了其在环境生物技术中对高效废水价值的适用性。

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来源期刊

Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

10.70

自引率

8.60%

发文量

846

审稿时长

24 days

期刊介绍： The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies