A dual-signal-amplified photoelectrochemical aptasensor for dual detection of β-lactoglobulin and Ara h1 allergens

IF 13.3 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-04-26 DOI:10.1016/j.cej.2025.162540

Shumin Zhang, Kexin Zou, Qingmin Chen, Jie Wei, Tianhui Jiao, Min Chen, Yi Xu, Quansheng Chen, Xiaomei Chen

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Abstract

Developing a fast, accurate, and simple detection method for food allergens is imperative to prevent food allergy. In this study, an on–off-on photoelectrochemical (PEC) aptasensor with dual-signal amplification was constructed based on a covalent organic frame (COF) film to detect β-lactoglobulin (BLG) and Ara h1. Specifically, a uniform and stable TFTA COF film was in situ grown as a PEC signifier on the surface of an indium tin oxide (ITO) electrode. BLG recognition triggered enzyme-assisted circular signal amplification to introduce non-conductive SiO₂-modified DNA (S0) to the platform, effectively quenching the PEC signal for ultra-sensitive detection of BLG. Upon adding Ara h1, SiO₂-DNA was released from the sensing platform to restore the PEC signal, and hemin was added to form a G-quadruplex/hemin compound for secondary signal amplification. Thus, sensitive detections of BLG and Ara h1 were achieved with low detection limits (18 ng/mL for BLG and 2.6 ng/mL for Ara h1) and wide linear ranges (0.05–5 µg/mL for BLG and 0.01–0.2 µg/mL for Ara h1). The dual-amplification and dual-target-responsive PEC aptasensor provides a new idea to develop PEC analysis methods for multi-targets in food and environmental monitoring.

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一种用于β-乳球蛋白和Ara h1过敏原双重检测的双信号放大光电感应传感器

开发一种快速、准确、简便的食物过敏原检测方法是预防食物过敏的必要手段。本研究基于共价有机框架（COF）膜构建了双信号放大的光电化学（PEC）感应传感器，用于检测β-乳球蛋白（BLG）和Ara h1。具体来说，在氧化铟锡（ITO）电极表面原位生长了一层均匀稳定的TFTA COF薄膜作为PEC标志。BLG识别触发酶辅助的环状信号放大，将非导电的sio2修饰DNA （S0）引入平台，有效地淬灭PEC信号，实现BLG的超灵敏检测。加入Ara h1后，传感平台释放SiO2-DNA恢复PEC信号，加入血红蛋白形成g -四重体/血红蛋白化合物进行二次信号放大。因此，BLG和Ara h1的检测灵敏度高，检测限低（BLG为18 ng/mL， Ara h1为2.6 ng/mL），线性范围宽（BLG为0.05-5 µg/mL， Ara h1为0.01-0.2 µg/mL）。双放大、双目标响应型PEC配体传感器为食品环境监测中多目标PEC分析方法的发展提供了新的思路。

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.