R. Medina, S. M. López, C. G. Lucentini, J. M. Reparaz, P. A. Balatti
{"title":"First Report of Crinivirus Tomatichlorosis Infecting Tomato Plants in Argentina","authors":"R. Medina, S. M. López, C. G. Lucentini, J. M. Reparaz, P. A. Balatti","doi":"10.1111/jph.70064","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Tomato is mainly produced in five main areas distributed in western, northern, eastern and south Argentina. In Jujuy, Argentina, tomato plants with yellowing, rolling, brittleness leaves symptoms were observed and samples were taken to the lab. Total RNA was extracted from symptomatic plants, and reverse transcription–PCR was done in order to detect simultaneously <i>Crinivirus tomatichlorosis</i>, <i>Crinivirus contagichlorosis.</i> Degenerate primers (HS-11/HS-12) and specific primers (TIC-3/TIC-4 and ToC-5/ToC-6) were used together to detect these two viruses in a nested-PCR. Only a PCR fragment of the expected size for <i>Crinivirus tomatichlorosis</i> (463 bp) was amplified from symptomatic diseased plants. The sequence of the amplicon blasted with <i>Crinivirus tomatichlorosis</i> virus protein (97.6%). Phylogenetic analysis supported this finding. So, here we report for the first time the <i>Crinivirus tomatichlorosis</i> infecting tomato cultivars in our country.</p>\n </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 2","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.70064","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Tomato is mainly produced in five main areas distributed in western, northern, eastern and south Argentina. In Jujuy, Argentina, tomato plants with yellowing, rolling, brittleness leaves symptoms were observed and samples were taken to the lab. Total RNA was extracted from symptomatic plants, and reverse transcription–PCR was done in order to detect simultaneously Crinivirus tomatichlorosis, Crinivirus contagichlorosis. Degenerate primers (HS-11/HS-12) and specific primers (TIC-3/TIC-4 and ToC-5/ToC-6) were used together to detect these two viruses in a nested-PCR. Only a PCR fragment of the expected size for Crinivirus tomatichlorosis (463 bp) was amplified from symptomatic diseased plants. The sequence of the amplicon blasted with Crinivirus tomatichlorosis virus protein (97.6%). Phylogenetic analysis supported this finding. So, here we report for the first time the Crinivirus tomatichlorosis infecting tomato cultivars in our country.
期刊介绍：
Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays.
Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes.
Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
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