UPLC-MS/MS analysis of Salvia miltiorrhiza Bge. Extract and its ameliorative effect of endothelial cell metabolism in isoproterenol-induced myocardial injury

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-04-18 DOI:10.1016/j.fitote.2025.106554

Yan Li, Xiaofei Xue, Meng Peng, Xiaolin Zheng, Hailong Tao, Sen Guo

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Abstract

Salvia miltiorrhiza Bge. (S. miltiorrhiza) is an important herbal medicine, and the researches mainly focused on the roots of S. miltiorrhiza rather than the above-ground parts. Therefore, the detailed research on the above-ground parts of S. miltiorrhiza is still unclear. This study aimed to elucidate the constituents of the above-ground parts of S. miltiorrhiza extract (SME) and evaluate its effect on endothelial cell injury. For this purpose, the constituents of SME were identified by ultra performance liquid chromatography tandem mass spectrometry, and 41 compounds were determined, including terpenes, phenylpropanoids, flavonoids, sterols and phenolic acids. The effect of SME on endothelial cell was evaluated using isoproterenol (ISO)-treated C57BL/6 mice and hypoxia-treated HUVECs, and the results revealed that SME could improve endothelial cell injury in ISO-induced myocardial infarction model and hypoxia-induced HUVECs injury. In this process, SME could inhibit reactive oxygen species production, decrease the levels of malondialdehyde, lactic dehydrogenase, pyruvic acid, intracellular iron ions and sugar, and increase the levels of glutathione. Meanwhile, SME could improve the abnormal expressions of apoptosis-, glycolysis- and ferroptosis-related proteins. Taken together, our data demonstrated that SME treatment could alleviate endothelial cell injury by regulating HIF-1α mediated glycolysis and ferroptosis signals.

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丹参的UPLC-MS/MS分析。异丙肾上腺素对心肌损伤内皮细胞代谢的改善作用

丹参。丹参（S. miltiorrhiza）是一种重要的中草药，研究主要集中在丹参的根部，而不是地上部分。因此，对丹参地上部分的详细研究尚不清楚。本研究旨在阐明丹参提取物地上部分的成分，并评价其对内皮细胞损伤的影响。为此，采用超高效液相色谱串联质谱法对其成分进行鉴定，共鉴定出萜类、苯丙类、黄酮类、甾醇类和酚酸类等41种化合物。采用异丙肾上腺素（ISO）处理的C57BL/6小鼠和缺氧处理的HUVECs，研究了SME对内皮细胞的影响，结果表明，SME可以改善ISO诱导的心肌梗死模型内皮细胞损伤和缺氧诱导的HUVECs损伤。在此过程中，SME能抑制活性氧的产生，降低丙二醛、乳酸脱氢酶、丙酮酸、细胞内铁离子和糖的水平，提高谷胱甘肽的水平。同时，SME可以改善细胞凋亡-、糖酵解-和铁中毒相关蛋白的异常表达。综上所述，我们的数据表明，SME治疗可以通过调节HIF-1α介导的糖酵解和铁下垂信号来减轻内皮细胞损伤。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.