Metabolism of sphingolipids in a rat spinal cord stenosis model

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Baasanjav Uranbileg , Yoko Hoshino , Mariko Ezaka , Makoto Kurano , Kanji Uchida , Yutaka Yatomi , Nobuko Ito
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引用次数: 0

Abstract

Background

Lumbar spinal canal stenosis (LSCS) plays a crucial role in neurogenic claudication and neuropathic pain. Recent studies suggest that changes in sphingolipid metabolism are linked to neuropathic pain. To explore the association between sphingolipids and LSCS, we measured the levels of sphingolipids and sphingolipid-associated molecules in an animal model of cauda equina compression (CEC), a typical type of LSCS.

Methods

By placing silicon blocks within the lumbar epidural space, CEC model were constructed in which motor disfunction had already been confirmed in our previous study. Quantitative measurements of various sphingolipids were conducted using LC-MS/MS in spinal cord and cerebrospinal fluid (CSF) samples on days 1, 7, and 28 following insertion of silicon blocks. Additionally, gene expression was analyzed in spinal cord tissue.

Results

In the CEC model, there was a significant increase ceramide levels in the CSF with upregulation of ceramide synthase 1 in the spinal cord tissue samples on day 1. Further, S1P levels in the CSF increased on day 7 and in the spinal cord significantly increased on day 28, and there was an increase in mRNA expression levels of sphingosine kinases (SphK)1 on days 1,7, and 28, while SphK2 on days 7 and 28. Regarding S1P receptors, there was an increase in mRNA expression levels of S1P1 on days 1,7, and 28 and S1P3 on day1.

Conclusion

The production and activation of the sphingolipid signaling pathway could play a pivotal role in neuropathic pain related to LSCS.
大鼠脊髓狭窄模型中鞘脂代谢的研究
背景:腰椎管狭窄(LSCS)在神经源性跛行和神经性疼痛中起着至关重要的作用。最近的研究表明鞘脂代谢的变化与神经性疼痛有关。为了探讨鞘脂与LSCS之间的关系,我们测量了马尾受压(CEC)动物模型中鞘脂和鞘脂相关分子的水平,CEC是LSCS的一种典型类型。方法通过在腰椎硬膜外间隙内放置硅块,建立运动功能障碍的CEC模型。在插入硅块后的第1、7和28天,使用LC-MS/MS对脊髓和脑脊液(CSF)样品中的各种鞘脂进行定量测量。此外,还分析了脊髓组织中的基因表达。结果在CEC模型中,脊髓组织样品中神经酰胺合成酶1在第1天上调,脑脊液中神经酰胺水平显著升高。此外,脑脊液中S1P水平在第7天升高,脊髓中S1P水平在第28天显著升高,鞘肽激酶(sphk1) mRNA表达水平在第1、7和28天升高,SphK2 mRNA表达水平在第7和28天升高。在S1P受体方面,S1P1 mRNA表达量在第1、7、28天升高,S1P3 mRNA表达量在第1天升高。结论鞘脂信号通路的产生和激活可能在LSCS相关神经性疼痛中起关键作用。
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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