Overexpression of the lectin receptor-like kinase gene OsLecRK-S.7 inhibits plant growth and enhances disease resistance in rice

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-04-22 DOI:10.1016/j.plantsci.2025.112517

Xiaoqun Peng , Yilin Li , Jingmei Xu , Ying Zeng , Kun Li , Xiangyi Guo , Zikang Zhang , Xiaoyan Tang , Menglong Wang

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引用次数: 0

Abstract

Lectin receptor-like kinases (LecRKs) are a critical class of plant proteins that play essential roles in plant development as well as in responses to both biotic and abiotic stresses. In this study, we found that overexpression of the L-type Lectin receptor kinase gene OsLecRK-S.7 severely inhibits plant growth and triggers spontaneous cell death. Meanwhile, immune responses, including pathogenesis-related (PR) gene expression and reactive oxygen species (ROS) accumulation, were elevated in OsLecRK-S.7 overexpressing plants. Kinase inactivation experiments demonstrated that kinase activity was essential for OsLecRK-S.7-mediated constitutive immunity. Infection assays further demonstrated that overexpression of OsLecRK-S.7 enhances rice resistance to bacterial blight. Additionally, bimolecular fluorescence complementation (BiFC) and pull-down experiments identified interactions between OsLecRK-S.7 and receptor-like cytoplasmic kinases (RLCKs) OsRLCK118, OsRLCK185, and OsRLCK107 that are involved in immune signaling. These findings suggest that OsLecRK-S.7 is a significant regulator of plant immunity, likely promoting cell death and immune responses through its interactions with OsRLCK118, OsRLCK185, and OsRLCK107.

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凝集素受体样激酶基因OsLecRK-S的过表达。7抑制植物生长，增强水稻抗病性

凝集素受体样激酶（LecRKs）是一类重要的植物蛋白，在植物发育以及对生物和非生物胁迫的反应中起着重要作用。本研究发现l型凝集素受体激酶基因OsLecRK-S过表达。7 .严重抑制植物生长，引发细胞自发死亡。同时，免疫应答，包括致病相关（PR）基因表达和活性氧（ROS）积累，在OsLecRK-S中升高。7 .过表达植物。激酶失活实验表明，激酶活性对OsLecRK-S至关重要。7介导的本构免疫。感染实验进一步证实了OsLecRK-S的过表达。7增强水稻对白叶枯病的抵抗力。此外，双分子荧光互补（BiFC）和下拉实验确定了OsLecRK-S之间的相互作用。7和受体样细胞质激酶（RLCKs） OsRLCK118、OsRLCK185和OsRLCK107参与免疫信号传导。这些发现表明OsLecRK-S。7是植物免疫的重要调节因子，可能通过与OsRLCK118、OsRLCK185和OsRLCK107的相互作用促进细胞死亡和免疫应答。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.