Compost Teas Reduce Verticillium dahliae Growth In Vitro via Direct and Volatile Effects

Hatem M. Younes, Summer R. A. Lockhart, Lynne Carpenter-Boggs
{"title":"Compost Teas Reduce Verticillium dahliae Growth In Vitro via Direct and Volatile Effects","authors":"Hatem M. Younes,&nbsp;Summer R. A. Lockhart,&nbsp;Lynne Carpenter-Boggs","doi":"10.1002/sae2.70067","DOIUrl":null,"url":null,"abstract":"<p>Verticillium wilt of potatoes is caused by the fungus <i>Verticillium dahliae</i>, which leads to significant economic losses in potato production. The fungus is soil-borne and can remain for up to 14 years in the soil or previous crop residues as hard-to-suppress microsclerotia. Compost teas (CTs) have been shown to suppress a variety of pathogenic fungi in food crops and are a sustainable option for pathogen suppression and crop nutrient provision. CTs are prepared by extracting compost in water and allowing it to brew for a period of time. In this study, we used a factorial experiment of two composts with four combinations of additives and aeration time to prepare eight CTs. Each CT was used after 3, 6 and 10 days of brewing. We tested the direct and volatile effects of unsterilised and filter-sterilised CTs on the mycelial growth of <i>V. dahliae</i> in vitro. Unsterilised CTs inhibited <i>V. dahliae</i> growth by 90.1% via direct suppression and 71.7% via volatile suppression. Sterilised CTs inhibited <i>V. dahliae</i> growth by 79.3% via direct suppression and 26.6% via volatile suppression. CT efficacy was least at 3 days of brewing time and greatest at 6 days. Scanning electron microscopy revealed mycoparasitism by several fungal species on <i>V. dahliae</i>, indicating that mycoparasitism may be a source of CT efficacy as a biocontrol agent. This study demonstrates that, with further development, CTs have the potential to be a sustainable solution for suppressing verticillium wilt in potatoes.</p>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"4 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.70067","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Sustainable Agriculture and Environment","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/sae2.70067","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Verticillium wilt of potatoes is caused by the fungus Verticillium dahliae, which leads to significant economic losses in potato production. The fungus is soil-borne and can remain for up to 14 years in the soil or previous crop residues as hard-to-suppress microsclerotia. Compost teas (CTs) have been shown to suppress a variety of pathogenic fungi in food crops and are a sustainable option for pathogen suppression and crop nutrient provision. CTs are prepared by extracting compost in water and allowing it to brew for a period of time. In this study, we used a factorial experiment of two composts with four combinations of additives and aeration time to prepare eight CTs. Each CT was used after 3, 6 and 10 days of brewing. We tested the direct and volatile effects of unsterilised and filter-sterilised CTs on the mycelial growth of V. dahliae in vitro. Unsterilised CTs inhibited V. dahliae growth by 90.1% via direct suppression and 71.7% via volatile suppression. Sterilised CTs inhibited V. dahliae growth by 79.3% via direct suppression and 26.6% via volatile suppression. CT efficacy was least at 3 days of brewing time and greatest at 6 days. Scanning electron microscopy revealed mycoparasitism by several fungal species on V. dahliae, indicating that mycoparasitism may be a source of CT efficacy as a biocontrol agent. This study demonstrates that, with further development, CTs have the potential to be a sustainable solution for suppressing verticillium wilt in potatoes.

Abstract Image

堆肥茶通过直接和挥发作用抑制大丽花黄萎病的体外生长
马铃薯黄萎病是由大丽花黄萎病真菌引起的,给马铃薯生产造成重大经济损失。这种真菌是土壤传播的,可以在土壤或以前的作物残留物中作为难以抑制的微菌核存活长达14年。堆肥茶(CTs)已被证明可以抑制粮食作物中的多种致病真菌,是抑制病原体和作物营养供应的可持续选择。ct是通过在水中提取堆肥并让其酿造一段时间来制备的。在本研究中,我们采用了两种堆肥,四种添加剂组合和曝气时间的析因试验,制备了8种ct。每个CT分别在3、6和10天后使用。我们在体外测试了未灭菌和过滤灭菌的ct对大丽花菌丝生长的直接和挥发性影响。未灭菌的ct通过直接抑制和挥发性抑制分别抑制了90.1%和71.7%的dahliae生长。灭菌后的ct通过直接抑制和挥发性抑制分别抑制了79.3%和26.6%的大丽花生长。CT的效果在冲泡时间3天最少,在冲泡时间6天最大。扫描电镜显示了几种真菌在大丽花上的分枝寄生,表明分枝寄生可能是CT作为生物防治剂效果的一个来源。这项研究表明,随着进一步的发展,ct有可能成为抑制马铃薯黄萎病的可持续解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
2.60
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信