Identification of a novel birnavirus strain in diseased Siniperca chuatsi and transcriptome profiling of virus-infected MFSB cells

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-04-22 DOI:10.1016/j.aquaculture.2025.742618

Hongye Cui , Haiyue Chen , Yuhui Wang , Yaoda Wang , Tianmei Hu , Liwen Xu , Baozhou Wu , Shuguang Hao , Xiong Hu , Jiping Zhang , Yu Liu , Weiwei Zeng

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引用次数: 0

Abstract

In August 2023, an outbreak of disease occurred in a pond containing Siniperca chuatsi fingerlings at a farm in Foshan, Guangdong province, resulting in a mortality rate exceeding 90 %. In this study, a novel birnavirus strain was isolated and identified from the diseased Siniperca chuatsi using cell culture, electron microscopy (EM), immunofluorescence assay (IFA), whole genome sequencing, and genetic evolutionary analysis. The virus was provisionally named Siniperca chuatsi Birnavirus FS202308 (SCBV-FS202308). Additionally, we analyzed the transcriptomic expression of SCBV-202308-infected mandarin fish spinal bulb (MFSB) cells. Results showed the MFSB cells inoculated with tissue homogenate exhibited typical cytopathic effects (CPE) post-infection. EM revealed a large number of icosahedral virion particles with a diameter approximately 60 nm were observed in the cells. Whole genome sequencing and phylogenetic analyses confirmed that the virus is a member of the family Birnavirus, specifically classified within the genus Blosnavirus. IFA utilizing antibodies against VP2 of SCBV and against IPNV revealed strong green fluorescent signals in the infected cells. Additionally, the challenge test demonstrated that all virus-inoculated S. chuatsi were successfully reinfected with SCBV-FS202308, resulting in a final mortality rate of approximately 20 %. Moreover, transcriptome analysis identified 2882 up-regulated and 3101 down-regulated genes in SCBV-FS202308-infected cells compared to the uninfected MFSB cells. Additionally, 522 genes were differentially enriched in pathways associated with immune signaling, such as the MAPK signaling, PI3K-Akt signaling, and Rap1 signaling pathways. This suggests that the virus elicits a robust immune response upon infecting host cells. This study represents the first report on the isolation, identification, and characterization of birnavirus from Siniperca chuatsi.

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一株新型冠状鳜病毒病株的鉴定及病毒感染的MFSB细胞的转录组分析

2023年8月，广东省佛山市一家养殖场的翘嘴鳜鱼种池发生疾病暴发，导致死亡率超过90%。本研究利用细胞培养、电镜、免疫荧光、全基因组测序和遗传进化分析等方法，从患病的翘嘴鳜中分离并鉴定了一株新型病毒。该病毒暂时命名为中华鳜伯纳病毒FS202308 （SCBV-FS202308）。此外，我们分析了scbv -202308感染鳜鱼脊髓球（MFSB）细胞的转录组表达。结果表明，组织匀浆接种的MFSB细胞感染后表现出典型的细胞病变效应（CPE）。电镜显示细胞内可见大量直径约60 nm的二十面体病毒粒子。全基因组测序和系统发育分析证实该病毒是伯纳病毒科的一员，具体归类为布洛斯纳病毒属。利用抗SCBV VP2抗体和抗IPNV抗体的IFA在感染细胞中显示出强烈的绿色荧光信号。此外，攻毒试验表明，所有接种过该病毒的猪链球菌都能成功地再次感染SCBV-FS202308，最终死亡率约为20%。此外，转录组分析发现，与未感染的MFSB细胞相比，感染scbv - fs202308的细胞中有2882个基因上调，3101个基因下调。此外，522个基因在与免疫信号通路相关的通路中存在差异富集，如MAPK信号通路、PI3K-Akt信号通路和Rap1信号通路。这表明该病毒在感染宿主细胞时引起强烈的免疫反应。本文首次报道了翘嘴鳜病毒的分离、鉴定和特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.