Nitric oxide detection by electrochemistry selective probe: calibration in the study environment is mandatory

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry Pub Date : 2025-04-19 DOI:10.1016/j.niox.2025.04.002

Roberta Albino Dos Reis , Ariane Boudier , Flavian Piquard , Joana C. Piereti , Amedea B. Seabra , Igor Clarot

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Abstract

Nitric oxide (NO) plays a crucial role in various physiological processes, making its detection and controlled release significant for both therapeutic and environmental contexts. Electrochemical sensors are widely used for NO detection due to their high sensitivity and real-time monitoring capabilities. However, challenges such as interference from other gasotransmitters, sensor degradation, and calibration difficulties—especially in complex biological matrices—hinder accurate NO measurement. This review discusses recent advancements in electrochemical NO detection, with a focus on the impact of complex biological matrices, calibration strategies, and sensor designs. The release of NO from nanoparticles, such as S-nitrosoglutathione (GSNO)-encapsulating chitosan nanoparticles, is used as a case study for improving NO detection accuracy. Future innovations in sensor technology and nanoparticle design are expected to expand the applicability of NO detection in personalized medicine and environmental monitoring.

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电化学选择性探针检测一氧化氮：在研究环境中校准是强制性的

一氧化氮（NO）在各种生理过程中起着至关重要的作用，使其检测和控制释放在治疗和环境背景下都具有重要意义。电化学传感器以其高灵敏度和实时监测能力被广泛应用于NO检测。然而，来自其他气体发射器的干扰、传感器退化和校准困难等挑战——特别是在复杂的生物基质中——阻碍了NO的准确测量。本文综述了电化学NO检测的最新进展，重点讨论了复杂生物基质、校准策略和传感器设计的影响。以s -亚硝基谷胱甘肽（GSNO）包封壳聚糖纳米颗粒为例，研究了纳米颗粒释放NO的方法，以提高NO检测的准确性。传感器技术和纳米颗粒设计的未来创新有望扩大一氧化氮检测在个性化医疗和环境监测中的适用性。

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来源期刊

Nitric oxide : biology and chemistry 生物-生化与分子生物学

CiteScore

7.50

自引率

7.70%

发文量

审稿时长

52 days

期刊介绍： Nitric Oxide includes original research, methodology papers and reviews relating to nitric oxide and other gasotransmitters such as hydrogen sulfide and carbon monoxide. Special emphasis is placed on the biological chemistry, physiology, pharmacology, enzymology and pathological significance of these molecules in human health and disease. The journal also accepts manuscripts relating to plant and microbial studies involving these molecules.