NRDN: A novel nuclear degradation tag for targeted protein regulation in Arabidopsis

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-04-24 DOI:10.1016/j.plantsci.2025.112529

Liting Shen , Huizhen Huang , Daqi Yan , Yongsheng Ye , Jun Hu

引用次数: 0

Abstract

The regulation of protein levels in plants is essential for improving agricultural productivity. Recent studies have explored inducible degradation systems in plants, with some showing promising advancements. This study introduces the NRDN degradation tag as a novel tool for regulating protein stability within the nucleus in Arabidopsis thaliana. Unlike traditional gene knockout methods, NRDN offers real-time, dynamic control over protein degradation, enabling precise studies of nuclear-localized proteins. This discovery provides a valuable tool for regulating protein stability in specific cellular compartments, which presents a versatile approach for dissecting complex cellular processes and offers broad applications in functional genomics and cellular research.

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NRDN：一种用于拟南芥靶向蛋白调控的新型核降解标签

植物体内蛋白质水平的调节对提高农业生产力至关重要。最近的研究探索了植物的诱导降解系统，其中一些显示出有希望的进展。本研究介绍了NRDN降解标签作为调节拟南芥细胞核内蛋白质稳定性的新工具。与传统的基因敲除方法不同，NRDN提供对蛋白质降解的实时、动态控制，使核定位蛋白的精确研究成为可能。这一发现为调节特定细胞区室的蛋白质稳定性提供了有价值的工具，为解剖复杂的细胞过程提供了一种通用的方法，并在功能基因组学和细胞研究中提供了广泛的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.