Microdissection testicular sperm extraction in male partner with non-obstructive azoospermia: Fresh or frozen–thawed sperm for intracytoplasmic sperm injection?

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research Pub Date : 2025-04-24 DOI:10.1111/jog.16298

Sevinc Özmen, Esra Nur Tola

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引用次数: 0

Abstract

Aim

To compare the outcomes of intracytoplasmic sperm injection (ICSI) of fresh and cryopreserved sperm obtained via microdissection testicular sperm extraction (micro-TESE) in cases of non-obstructive azoospermia (NOA).

Methods

A total of 147 patients with NOA who underwent micro-TESE and obtained viable sperm via micro-TESE at Istanbul Medipol In Vitro Fertilization unit were recruited retrospectively. The cases were divided into two groups depending on the sperm used (fresh or frozen–thawed) for ICSI: The micro-TESE group (n = 93) underwent ICSI cycles with fresh spermatozoa and the micro-TESE-Thaw group (n = 54) underwent ICSI cycles with cryopreserved spermatozoa. Patient demographics, embryo development, and pregnancy outcomes were compared between the two groups.

Results

No statistical difference was observed between the two groups in terms of demographic features (age, body mass index, etc.) and cycle characteristics (induction protocol, gonadotropin dose, etc.). Fertilization rates and embryo quality were also similar between the groups. Higher clinical pregnancy and live birth rates were observed in the micro-TESE group than in the micro-TESE-Thaw group (p <0.04 and p <0.003, respectively). The miscarriage rate was higher in the micro-TESE-Thaw group, although the difference did not reach statistical significance.

Conclusion(s)

In cases where frozen sperm obtained by micro-TESE are used, even if viable and motile sperm are found after thawing, there may be a negative impact on the ICSI result. Fresh testicular spermatozoa appear to result in better clinical pregnancy and live birth rates than cryopreserved testicular spermatozoa in males with NOA.

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非阻塞性无精子症男性伴侣的显微解剖睾丸精子提取：新鲜精子还是冷冻解冻精子用于胞浆内单精子注射？

目的比较非阻塞性无精子症（NOA）患者通过显微解剖睾丸精子提取（micro-TESE）获得的新鲜精子和冷冻精子卵胞浆内单精子注射（ICSI）的效果。方法回顾性分析伊斯坦布尔Medipol体外受精中心147例NOA患者，经微创tese手术获得活精子。根据使用的精子（新鲜或冷冻解冻）将病例分为两组：使用新鲜精子进行ICSI的micro-TESE组（n = 93）和使用冷冻保存的精子进行ICSI的micro- tse - thaw组（n = 54）。比较两组患者的人口统计、胚胎发育和妊娠结局。结果两组在人口统计学特征（年龄、体质指数等）和周期特征（诱导方案、促性腺激素剂量等）方面无统计学差异。各组受精率和胚胎质量相似。微tese组临床妊娠率和活产率高于微tse - thaw组（p <；0.04和p <；0.003）。微tse - thaw组流产率较高，但差异无统计学意义。结论(5)使用微低温冷冻获得的冷冻精子，即使在解冻后发现有活力和活动的精子，也可能对ICSI结果产生负面影响。在男性NOA患者中，新鲜睾丸精子比冷冻保存的睾丸精子有更好的临床妊娠和活产率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Obstetrics and Gynaecology Research 医学-妇产科学

CiteScore

3.10

自引率

0.00%

发文量

376

审稿时长

3-6 weeks

期刊介绍： The Journal of Obstetrics and Gynaecology Research is the official Journal of the Asia and Oceania Federation of Obstetrics and Gynecology and of the Japan Society of Obstetrics and Gynecology, and aims to provide a medium for the publication of articles in the fields of obstetrics and gynecology. The Journal publishes original research articles, case reports, review articles and letters to the editor. The Journal will give publication priority to original research articles over case reports. Accepted papers become the exclusive licence of the Journal. Manuscripts are peer reviewed by at least two referees and/or Associate Editors expert in the field of the submitted paper.