Electrochemical Immunosensing of The Neo-Antigen Collagen Type I α 1 to Assist in the Personalized Management of Advanced Colorectal Cancer

Abstract

Biomarkers known as neo-antigens, such as collagen, are increasingly considered of interest for the diagnosis and therapeutic monitoring of oncology patients. This work reports the development of an attractive strategy for the amperometric immunodetection of Collagen type I α 1 (COL1A1), involving magnetic microbeads for the construction of the immunoplatform, a sandwich assay format using specific capture and biotinylated-detector antibodies, horseradish peroxidase labeling, and amperometric transduction at disposable screen-printed carbon electrodes. The developed methodology allowed the selective, rapid, simple, and reliable detection of COL1A1 in the 0.051–10 ng mL⁻¹ linear range with a detection limit of 15 pg mL⁻¹. The developed immunoplatform was successfully applied to the analysis of COL1A1 in plasma and tissue extracts from healthy individuals and colorectal cancer patients. The results obtained proved the potential of this neo-antigen as biomarker for diagnosis of this disease.