Biological applications on a novel composite hydrogel containing mesoporous silica nanoparticles loaded with insulin-like growth Factor-1 and Vancomycin

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2025-04-25 DOI:10.1007/s10856-025-06884-5

Haiyong Ren, Qiaofeng Guo, Bingyuan Lin, Yiyang Liu, Xiang Wang, Kai Huang, Kaiting Wu

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Abstract

Polymer nanocomposites have prolific applications in the environmental and health domain. Healthcare workers have constantly faced a challenge in constructing an effective sustained-release system for drugs, especially in areas where it is difficult to follow traditional treatment guidelines. To achieve a dual sustained release of vancomycin and cell growth factors, we designed a composite hydrogel, which was polymerized under ultraviolet irradiation to form a methacrylate gelatin (GelMA)/polyethylene glycol diacrylate (PEGDA) cross-linked system. The hydrogel showed antibacterial and pro-osteogenic differentiation activities by blending it with vancomycin and insulin-like growth factor 1 (IGF-1)-loaded silica particles. The experimental results showed that with increased PEGDA concentration, the hydrogels were degraded by swelling with a longer sustained release time. After 144-h immersion, the hydrogel still showed sustained release of IGF-1. The antibacterial experiment showed that the hydrogel pair had an obvious antibacterial effect against S. aureus. The results of live/dead staining and Cell Counting kit-8 assay indicated excellent biocompatibility of the hydrogel. Alizarin Red staining (ARS) further demonstrated that this hydrogel could induce bone marrow stem cells to initiate osteogenic differentiation. This hydrogel material exhibited excellent antibacterial and pro-osteogenic differentiation properties and might be promising for chronic osteomyelitis treatment.

Graphical Abstract

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载胰岛素样生长因子-1和万古霉素的介孔二氧化硅纳米颗粒复合水凝胶的生物学应用

高分子纳米复合材料在环境和健康领域有着广泛的应用。构建有效的药物缓释系统一直是医疗工作者面临的挑战，特别是在难以遵循传统治疗指南的地区。为了实现万古霉素和细胞生长因子的双重缓释，我们设计了一种复合水凝胶，在紫外线照射下聚合形成甲基丙烯酸明胶(GelMA)/聚乙二醇二丙烯酸酯（PEGDA）交联体系。通过与万古霉素和负载胰岛素样生长因子1 （IGF-1）的二氧化硅颗粒混合，水凝胶显示出抗菌和促骨分化活性。实验结果表明，随着PEGDA浓度的增加，水凝胶发生溶胀降解，且缓释时间延长。浸泡144 h后，水凝胶仍显示IGF-1的缓释。抗菌实验表明，该水凝胶对金黄色葡萄球菌具有明显的抗菌作用。活/死染色和细胞计数试剂盒-8实验结果表明，水凝胶具有良好的生物相容性。茜素红染色（ARS）进一步证实该水凝胶可诱导骨髓干细胞启动成骨分化。这种水凝胶材料具有良好的抗菌和促骨分化特性，有望用于慢性骨髓炎的治疗。图形抽象

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.