Single amino acid exchanges affect the substrate preference of an acetaldehyde dehydrogenase

IF 3.9 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Microbiology and Biotechnology Pub Date : 2025-04-25 DOI:10.1007/s00253-025-13468-4

Dominik Hege, Yvonne Gemmecker, Iris Schall, Paula Oppong-Nti, Georg Schmitt, Johann Heider

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引用次数: 0

Abstract

The NAD⁺-dependent aldehyde dehydrogenase AldB from Aromatoleum aromaticum was recombinantly produced in Escherichia coli and biochemically characterized. As suggested by its substrate spectrum, the most probably physiological function of AldB is the oxidation of short aliphatic aldehydes such as acetaldehyde, which occur as intermediates in the degradation of the corresponding alcohols. In addition, we generated some mutant variants in residue Tyr460, which is located at the neck region of the substrate channel and analyzed their effects on the catalytic parameters for different substrates. Single amino acid exchanges at this position revealed profound changes in substrate preference and substrate inhibition of the variants.

• Small aliphatic aldehydes show the best catalytic efficiency with aldehyde dehydrogenase AldB

• Amino acid exchanges at Y460 results in changed catalytic efficiencies and substrate inhibition

• AldB is a member of a new clade of the aldehyde dehydrogenase superfamily

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单氨基酸交换影响乙醛脱氢酶的底物偏好

在大肠杆菌中重组产生了依赖NAD+的Aromatoleum aromaticum醛脱氢酶AldB，并对其进行了生化鉴定。根据其底物光谱，AldB最可能的生理功能是氧化短脂肪醛，如乙醛，它在相应醇的降解过程中作为中间体出现。此外，我们在位于底物通道颈部的残基Tyr460中生成了一些突变变体，并分析了它们对不同底物催化参数的影响。该位置的单氨基酸交换揭示了底物偏好和底物抑制变异的深刻变化。•小脂肪族醛对醛脱氢酶AldB的催化效率最好•氨基酸在Y460处交换会改变催化效率和底物抑制•AldB是醛脱氢酶超家族的新分支的成员

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来源期刊

Applied Microbiology and Biotechnology 工程技术-生物工程与应用微生物

CiteScore

10.00

自引率

4.00%

发文量

535

审稿时长

2 months

期刊介绍： Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.