{"title":"Biological characteristics and genome analysis of Citrobacter freundii phage K1M","authors":"Keming Xie, Chang Liu, Guangfeng Liu, Peng Zhu, Zheng Yang, Lihong Yuan, Jingzhe Jiang","doi":"10.1007/s00705-025-06291-9","DOIUrl":null,"url":null,"abstract":"<div><p>Bacteriophages that infect specific bacteria and cause their lysis are potentially useful for preventing and treating bacterial infections and controlling microbes in the environment. <i>Citrobacter freundii</i> is an opportunistic pathogen that is commonly present in the environment, food, animals, and human intestines. It also causes aquatic animal diseases in aquaculture. In this study, using <i>Citrobacter freundii</i> as the target host, a novel lytic phage, vB_CfrS_K1M (K1M), was isolated. Morphologically, K1M is a long-tailed phage with a head diameter of 68 ± 2 nm and a tail length of 130 ± 2 nm. The phage encodes a polysaccharide depolymerase and demonstrates stable biological activity. The genome of K1M is 47,995 bp in length, with 45.12% G + C content and 97 putative open reading frames (ORFs), only 36 of which encode proteins. The results of phylogenetic analysis based on TerL sequences suggest that K1M represents a new viral genus, for which we propose the name \"<i>Fredivirus</i>\".</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 6","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s00705-025-06291-9","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Bacteriophages that infect specific bacteria and cause their lysis are potentially useful for preventing and treating bacterial infections and controlling microbes in the environment. Citrobacter freundii is an opportunistic pathogen that is commonly present in the environment, food, animals, and human intestines. It also causes aquatic animal diseases in aquaculture. In this study, using Citrobacter freundii as the target host, a novel lytic phage, vB_CfrS_K1M (K1M), was isolated. Morphologically, K1M is a long-tailed phage with a head diameter of 68 ± 2 nm and a tail length of 130 ± 2 nm. The phage encodes a polysaccharide depolymerase and demonstrates stable biological activity. The genome of K1M is 47,995 bp in length, with 45.12% G + C content and 97 putative open reading frames (ORFs), only 36 of which encode proteins. The results of phylogenetic analysis based on TerL sequences suggest that K1M represents a new viral genus, for which we propose the name "Fredivirus".
期刊介绍：
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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