High resolution two photon fluorescence probe monitoring ClO− based on anion exchange for the synergistic ROS and ferroptosis activated by thermal energy

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-20 DOI:10.1016/j.saa.2025.126277

Yitong Lin , Yan Pu , Xingchen Wang , Qiong Zhang , Haifeng Tang , Baocheng Jin , Ming Cao , Yan Feng , Xingxing Chen , Xiaojiao Zhu , Hongping Zhou

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Abstract

Ferroptosis, closely correlated with hypochlorite (ClO⁻), is an emerging form of iron-dependent cell death. Exploring the ClO⁻ content in living cells will help to further reveal the biological function of ferroptosis. In this work, we have developed a two-photon excited fluorescent probe (CMI-ClO) which can detect the ClO⁻ changes during ferroptosis. CMI-ClO can specifically response to ClO⁻ by exchanging with I⁻. The multiple reactive oxygen species (ROS) are produced simultaneously in the ClO⁻ identification process. More significantly, CMI-ClO produces ROS through thermal energy, breaking the limitation of light trigged conventional photosensitizers. CMI-ClO can achieve high resolution tracking of ferroptosis under NIR II excitation, where ROS and ferroptosis combine to cause mitochondrial damage and cell death. This work provides a synergistic strategy for enhancing ROS and ferroptosis therapy.

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基于阴离子交换的高分辨率双光子荧光探针监测ClO−的协同ROS和热能激活的铁下垂

铁下垂与次氯酸盐（ClO−）密切相关，是一种新兴的铁依赖性细胞死亡形式。研究活细胞中ClO−的含量有助于进一步揭示铁下垂的生物学功能。在这项工作中，我们开发了一种双光子激发荧光探针（CMI-ClO），可以检测铁下垂过程中ClO−的变化。CMI-ClO可以通过与I−交换来特异性响应ClO−。多种活性氧（ROS）在ClO -识别过程中同时产生。更重要的是，CMI-ClO通过热能产生ROS，打破了光触发传统光敏剂的限制。CMI-ClO可以在NIR II激发下实现高分辨率的铁下垂跟踪，其中ROS和铁下垂共同导致线粒体损伤和细胞死亡。这项工作为增强ROS和铁下垂治疗提供了协同策略。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.