Molecular events responsible for hypoglycaemic attributes of Fernandoa adenophylla extract in streptozotocin-induced diabetes

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-04-19 DOI:10.1016/j.fitote.2025.106557

Ammara Hakeem , Mohammad Saleem , Rukhsana Anwar , Muhammad Asif , Taha Muhammad , Sherif S. Ebada , Nawal M. Al Musayeib , Mohamed L. Ashour

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Abstract

Traditionally, people use the decoction of Fernandoa adenophylla leaves to treat diabetes mellitus. This study investigated the antidiabetic activity of F. adenophylla methanol extract (Fa.Me) in streptozotocin (STZ)-induced diabetes rats, with metformin as a positive control. We analysed several relevant biomarkers and antioxidant enzymes using ELISA assays and assessed gene expression (PEPCK, G6-PDH, PFKFB1, GS, GK, and GLUT-4) using qRT-PCR. The obtained results revealed an upregulation of G6-PDH (PPS pathway), PFKFB1 (glycolysis), GS (glycogenesis), and GLUT-4 (glucose transporter), while the PEPCK (gluconeogenesis) gene was downregulated. Besides, the antidiabetic biomarkers showed elevated adiponectin, SOD, and glutathione levels; however, amylase level decreased in the treatment groups. We observed a dose-dependent reduction in HbA1c, insulin, and random blood glucose in the metformin and Fa.Me groups. Histopathological analysis of animals' liver and pancreas showed an intact structure of hepatocytes in extract groups, while atrophic changes in the pancreas were observed in the diseased group. By modulating the oxidative stress and glucose metabolism-control genes, F. adenophylla demonstrated significant antidiabetic properties and held potential for further clinical exploration.

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在链脲佐菌素诱导的糖尿病中，腺茶树提取物的低血糖特性的分子事件

传统上，人们用茶树叶煎剂治疗糖尿病。以二甲双胍为阳性对照，研究了腺茶树甲醇提取物（Fa.Me）对链脲佐菌素（STZ）诱导的糖尿病大鼠的抗糖尿病活性。我们使用ELISA分析了几种相关的生物标志物和抗氧化酶，并使用qRT-PCR评估了基因表达（PEPCK、G6-PDH、PFKFB1、GS、GK和GLUT-4）。结果显示，G6-PDH （PPS通路）、PFKFB1（糖酵解）、GS（糖生成）和GLUT-4（葡萄糖转运蛋白）表达上调，而PEPCK（糖异生）基因表达下调。此外，抗糖尿病生物标志物显示脂联素、SOD和谷胱甘肽水平升高；然而，在治疗组，淀粉酶水平下降。我们观察到二甲双胍和Fa组的HbA1c、胰岛素和随机血糖呈剂量依赖性降低。我组。动物肝脏和胰腺组织病理学分析显示，提取物组肝细胞结构完整，而病变组胰腺出现萎缩变化。通过调节氧化应激和葡萄糖代谢控制基因，腺茶树显示出显著的抗糖尿病特性，并具有进一步临床探索的潜力。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.