Selection and characterization of acid-tolerant Chlorophyta division microalgae for potential metabolites production in a bubble column bioreactor: A ready-to-industrial scale screening

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-04-21 DOI:10.1016/j.biortech.2025.132579

Luigi Marra , Elena Aurino , Francesca Raganati , Antonino Pollio , Antonio Marzocchella

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Abstract

Microalgae are promising candidates for sustainable bioprocesses owing to their ability to capture/utilize CO₂ and produce valuable metabolites. However, strain-to-strain variability poses a challenge to industrial scalability. This paper reports a study on the screening of 28 Chlorophyta microalgal strains from six species cultivated under standardized semi-continuous conditions in a bubble column bioreactor. Growth kinetics, nitrogen uptake, and metabolite accumulation were analyzed to identify strains characterized by promising biomass/metabolite productivities.

The results indicated two distinct kinetic behaviors: a large fraction of the investigated strains was characterized by a common biomass/nitrate uptake behavior, and a subset of the strain pool was characterized by atypical nitrogen assimilation patterns. Strains characterized by high biomass growth rates are characterized by high lipid productivity. Strains with intermediate biomass growth rates are characterized by high carbohydrate accumulation. Chlorella vulgaris (ACUF 058) and Chloroidium saccharophilum (ACUF 050) emerged as strong candidates for carbohydrate and protein production, respectively.

This study proposed a systematic procedure for strain selection, bridging laboratory screening, and industrial feasibility. These results emphasize the role of tailored cultivation strategies in optimizing biomass yield and metabolite productivity. Further research integrating genomic and proteomic insights will enhance strain-specific process optimization, facilitating the large-scale deployment of microalgal bioprocesses.

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气泡柱生物反应器中用于潜在代谢物生产的耐酸绿藻分裂微藻的选择和特性：准备工业规模的筛选

微藻具有捕获/利用二氧化碳和产生有价值的代谢物的能力，是可持续生物过程的有希望的候选者。然而，应变对应变的可变性对工业可扩展性提出了挑战。本文报道了在标准化半连续条件下，在气泡柱生物反应器中从6种藻类中筛选28株绿藻微藻的研究。通过对生长动力学、氮吸收和代谢物积累进行分析，鉴定出具有良好生物量/代谢物生产力的菌株。结果表明：大部分菌株具有共同的生物量/硝酸盐吸收行为，而一小部分菌株库具有非典型的氮同化模式。具有高生物量生长率的菌株具有高脂质产量的特点。生物量增长率中等的菌株具有高碳水化合物积累的特点。小球藻（Chlorella vulgaris, ACUF 058）和嗜糖绿球藻（Chloroidium saccharophilum, ACUF 050）分别被认为是碳水化合物和蛋白质生产的有力候选者。本研究提出了一套系统的菌株选择、衔接实验室筛选和工业可行性的程序。这些结果强调了量身定制的栽培策略在优化生物量产量和代谢物生产力方面的作用。整合基因组学和蛋白质组学的进一步研究将增强菌株特异性工艺优化，促进微藻生物工艺的大规模部署。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.