Generation of two induced pluripotent stem cell lines from breast cancer patients carrying BRCA1 mutations

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-04-18 DOI:10.1016/j.scr.2025.103716

Yi-Ing Chen , Arianne Caudal , Amira G. Flores-Banuelos , Christopher D. Yan , Walter G. Park , Mohan Viswanathan , Joseph C. Wu

引用次数: 0

Abstract

Haploinsufficiency of BRCA1 increases the risk of various cancers due to its critical functions in cell cycle checkpoint regulation and DNA repair. This study generated and characterized two induced pluripotent stem cell (iPSC) lines derived from breast cancer patients with mutations in the BRCA1 gene (c.676del and c.5096G>A). Both lines exhibited typical iPSC morphology, karyotype, pluripotency marker expression, and trilineage differentiation capabilities. BRCA1-mutated iPSCs provide a valuable resource to investigate pathogenic mechanisms and develop personalized medicine.

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来自携带BRCA1突变的乳腺癌患者的两种诱导多能干细胞系的产生

由于其在细胞周期检查点调节和DNA修复中的关键功能，BRCA1的单倍性不足增加了各种癌症的风险。本研究从BRCA1基因（c.676del和c.5096G>；A）突变的乳腺癌患者中产生并鉴定了两种诱导多能干细胞（iPSC）系。两系均表现出典型的iPSC形态、核型、多能性标记表达和三龄分化能力。brca1突变的iPSCs为研究致病机制和开发个性化医疗提供了宝贵的资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.