Characterization of the Tuta absoluta virome reveals higher viral diversity in field populations

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology Pub Date : 2025-04-21 DOI:10.1016/j.jip.2025.108340

Rosa Esmeralda Becerra-García, Luis Hernández-Pelegrín, Cristina M Crava, Salvador Herrero

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引用次数: 0

Abstract

A significant number of insect-specific viruses (ISVs) have been discovered in agriculturally important insect pests, facilitated by high-throughput sequencing (HTS). Despite its global impact on tomato crops, the RNA virome of the South American tomato pinworm, Tuta absoluta, remains uncharacterized. In this study, we utilized meta-transcriptomics and bioinformatic approaches to discover the RNA virome of T. absoluta across worldwide populations. We identified ten novel ISVs, classified into eight groups: Nidovirales, Bunyavirales, Mononegavirales, Virgaviridae, Iflaviridae, Nodaviridae, Solemoviridae, and Phasmaviridae. Notably, no core virus was consistently present across the studied populations, and field-collected samples revealed a greater diversity of ISVs compared to those from laboratory samples. In addition, we detected plant-infecting viruses and mycoviruses associated with the pest. This study represents the first description of the RNA virome associated with T. absoluta, providing valuable insights into its biological and ecological interactions. It also lays the foundation for future studies aimed to clarify the biological roles of ISVs.

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绝对图塔病毒组的特征揭示了野外种群中较高的病毒多样性

利用高通量测序（HTS）技术，在重要的农业害虫中发现了大量昆虫特异性病毒（isv）。尽管南美番茄蛲虫（Tuta absoluta）对番茄作物产生了全球性的影响，但它的RNA病毒仍未被发现。在这项研究中，我们利用元转录组学和生物信息学方法发现了世界范围内绝对T.的RNA病毒。我们鉴定出10种新的独立病毒，分为8个类群：尼多病毒科、布尼亚病毒科、单奈加病毒科、处女病毒科、伊黄病毒科、诺达病毒科、索莱莫病毒科和Phasmaviridae。值得注意的是，在研究人群中没有核心病毒始终存在，现场收集的样本显示，与实验室样本相比，isv的多样性更大。此外，我们还检测到与该害虫相关的植物侵染病毒和分枝病毒。该研究首次描述了与T. absoluta相关的RNA病毒组，为其生物学和生态学相互作用提供了有价值的见解。这也为进一步阐明isv的生物学作用奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of invertebrate pathology 生物-动物学

CiteScore

6.10

自引率

5.90%

发文量

审稿时长

1 months

期刊介绍： The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.