The protective effect of bromelain on testicular dysfunction and oxidative stress induced by REM sleep deprivation in adult male rats

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-04-22 DOI:10.1016/j.tice.2025.102927

Mahboubeh Hosseinpour , Amir Masoud Dalband , Zahrasadat Lajevardi , Arezou Soltanattar , Armin Tafazolimoghadam , Amin Karamian , Mohammad-Amin Abdollahifar , Hojjat-Allah Abbaszadeh , Mahshid Hodaeipour , Ali Charmchi , Aliasghar Keramatinia , Armin Alinezhad , Fatemeh Zolfaghari , Mobina Fathi , Kimia Vakili , Fatemeh Navaei , Mojtaba Sani , Pourya Raee , Abbas Aliaghaei

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引用次数: 0

Abstract

Male infertility is a significant global health concern, with sleep deprivation emerging as a potential contributing factor due to its impact on oxidative stress and reproductive function. This study investigates the protective effects of bromelain, a natural anti-inflammatory enzyme, on testicular dysfunction induced by Rapid eye movement (REM) sleep deprivation in adult male rats. Thirty-six rats were divided into control, REM sleep-deprived (REM-dep), and REM-deprived with bromelain treatment (REM-dep+Br) groups. REM sleep deprivation was induced for a 21-day period using the flower pot technique, with concurrent oral administration of bromelain (200 mg/kg) throughout this period. Finally, sperm parameters, testicular stereology, antioxidant markers (Nrf-2, γ-GCS, and GSH), and inflammatory/apoptotic markers (TNF-α, and Caspase-3) were evaluated across study groups. REM sleep deprivation significantly reduced sperm count, motility (total, and progressive), and normal morphology while increasing DNA fragmentation compared to control group. Testicular stereology revealed decreased spermatogenic cells, Sertoli cells, Leydig cells, and Johnsen scores. Bromelain administration significantly improved total sperm motility, increased spermatid numbers, and restored Leydig cell counts compared to REM-dep group. Antioxidant markers (Nrf-2, γ-GCS, GSH) were significantly reduced in the REM-dep group but restored with bromelain treatment. Immunohistochemistry showed elevated TNF-α and Caspase-3 expression in the REM-dep group, which was mitigated by bromelain administration. These findings suggest that REM sleep deprivation induces testicular dysfunction through oxidative stress and inflammation, while bromelain exerts protective effects via its antioxidant and anti-inflammatory properties. This study highlights bromelain as a potential protective /therapeutic agent for mitigating sleep deprivation-induced male reproductive dysfunction.

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菠萝蛋白酶对成年雄性大鼠快速眼动睡眠剥夺所致睾丸功能障碍和氧化应激的保护作用

男性不育是一个重大的全球健康问题，由于睡眠不足对氧化应激和生殖功能的影响，它成为一个潜在的促成因素。研究天然抗炎酶菠萝蛋白酶对快速眼动（REM）睡眠剥夺致成年雄性大鼠睾丸功能障碍的保护作用。36只大鼠分为对照组、快速眼动睡眠剥夺组（REM- deep）和快速眼动睡眠剥夺加菠萝蛋白酶组（REM- deep +Br）。采用花盆技术诱导快速眼动睡眠剥夺21天，并在此期间同时口服菠萝蛋白酶（200 mg/kg）。最后，对各研究组的精子参数、睾丸体视学、抗氧化标志物（Nrf-2、γ-GCS和GSH）和炎症/凋亡标志物（TNF-α和Caspase-3）进行评估。与对照组相比，快速眼动睡眠剥夺显著减少了精子数量、活力（总活力和进行性）和正常形态，同时增加了DNA断裂。睾丸体视学显示生精细胞、支持细胞、间质细胞和Johnsen评分降低。与rem - deep组相比，菠萝蛋白酶显著提高了总精子活力，增加了精子数量，恢复了间质细胞计数。抗氧化标志物（Nrf-2、γ-GCS、GSH）在rem - deep组显著降低，但经菠萝蛋白酶处理后恢复。免疫组织化学显示rem - deep组TNF-α和Caspase-3表达升高，菠萝蛋白酶缓解了这种升高。这些发现表明，快速眼动睡眠剥夺通过氧化应激和炎症诱导睾丸功能障碍，而菠萝蛋白酶通过其抗氧化和抗炎特性发挥保护作用。这项研究强调菠萝蛋白酶是一种潜在的保护/治疗药物，可以缓解睡眠不足引起的男性生殖功能障碍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.